Function of SPI-6 T6SS and SPI-19 T6SS of Salmonella during competition with the gut microbiota

• Grant number: 18/25316-4
• Support type: Scholarships in Brazil - Scientific Initiation
• Effective date (Start): February 01, 2019
• Effective date (End): December 31, 2021
• Field of knowledge: Biological Sciences - Microbiology - Biology and Physiology of Microorganisms
• Principal researcher: Ethel Bayer Santos
• Grantee: Julia Takuno Hespanhol
• Home Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated research grant: 17/02178-2 - Function of type VI secretion systems of pathogenic bacteria in the interaction with eukaryotic cells, AP.JP

Abstract

The genome of Salmonella Typhimurium encodes a type six secretion system (T6SS) within the Salmonella-pathogenicity island 6 (SPI-6). In Salmonella Gallinarum, the T6SS is encoded within SPI-19. Curiously, Salmonella Dublin harbors two T6SSs (SPI-6 and SPI-19). All these systems are important for the establishment of infection in mice and chickens. Recently, it was shown that SPI-6 T6SS from S. Typhimurium is important for competition with bacterial species of the gut microbiota. It is unknown whether SPI-6 T6SS from other Salmonella serotypes or the SPI-19 T6SS would also function during bacterial competition. To clarity these questions, we intend to construct mutant strains for key structural components of T6SSs e test their fitness during competition with Escherichia coli. It is expected at the end of this work to clarify which stereotype and T6SS loci act against bacterial species.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SIBINELLI-SOUSA, STEPHANIE; HESPANHOL, JULIA T.; NICASTRO, GIANLUCCA G.; MATSUYAMA, BRUNO Y.; MESNAGE, STEPHANE; PATEL, ANKUR; DE SOUZA, ROBSON F.; GUZZO, CRISTIANE R.; BAYER-SANTOS, ETHEL. A Family of T6SS Antibacterial Effectors Related to L,D-Transpeptidases Targets the Peptidoglycan. CELL REPORTS, v. 31, n. 12 JUN 23 2020. Web of Science Citations: 0.