The genome of Salmonella Typhimurium encodes a type six secretion system (T6SS) within the Salmonella-pathogenicity island 6 (SPI-6). In Salmonella Gallinarum, the T6SS is encoded within SPI-19. Curiously, Salmonella Dublin harbors two T6SSs (SPI-6 and SPI-19). All these systems are important for the establishment of infection in mice and chickens. Recently, it was shown that SPI-6 T6SS from S. Typhimurium is important for competition with bacterial species of the gut microbiota. It is unknown whether SPI-6 T6SS from other Salmonella serotypes or the SPI-19 T6SS would also function during bacterial competition. To clarity these questions, we intend to construct mutant strains for key structural components of T6SSs e test their fitness during competition with Escherichia coli. It is expected at the end of this work to clarify which stereotype and T6SS loci act against bacterial species.
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