Analysis of the activation dynamics of the type 6 secretion system of Salmonella during infection of eukaryotic cells

• Grant number: 18/13819-1
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Effective date (Start): August 01, 2018
• Effective date (End): December 31, 2019
• Field of knowledge: Biological Sciences - Microbiology - Biology and Physiology of Microorganisms
• Principal Investigator: Ethel Bayer Santos
• Grantee: Stephanie Sibinelli de Sousa
• Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated research grant: 17/02178-2 - Function of type VI secretion systems of pathogenic bacteria in the interaction with eukaryotic cells, AP.JP

Abstract

The genome of Salmonella Typhimurium encodes a type VI secretion system (T6SS) inside the Salmonella pathogenicity island (SPI-6). In Salmonella Gallinarum the T6SS is encoded from SPI-19. Genes located inside SPI-6 T6SS from S. Typhimurium are activated during late stages of infection, while SPI-19 T6SS from S. Gallinarum are activated early during the infection of macrophages. The significance of the T6SS activation under these conditions is still unknown. To obtain further knowledge about the activation dynamics and the assembly of SPI-6 and SPI-19 T6SS, we plan to make bacterial strains expressing a structural T6SS component in phusion with superfolder GFP (sfGFP) to follow at the single cell level its assembly during the infection of different eukaryotic cell lines. At the end of the project, we expect to have a broader and integrated view about the activation of T6SSs during the intracellular stage of infection.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SIBINELLI-SOUSA, STEPHANIE; HESPANHOL, JULIA T.; NICASTRO, GIANLUCCA G.; MATSUYAMA, BRUNO Y.; MESNAGE, STEPHANE; PATEL, ANKUR; DE SOUZA, ROBSON F.; GUZZO, CRISTIANE R.; BAYER-SANTOS, ETHEL. A Family of T6SS Antibacterial Effectors Related to L,D-Transpeptidases Targets the Peptidoglycan. CELL REPORTS, v. 31, n. 12, JUN 23 2020. (18/04553-8, 17/02178-2, 16/09047-8, 19/00195-2, 17/17303-7, 18/25316-4, 16/00458-5, 18/13819-1)