An aptasensing platform for detection of heat shock protein 70 kDa (HSP70) using a modified gold electrode with lady fern-like gold (LFG) nanostructure

Author(s)

Negahdary, Masoud ; Angnes, Lucio

• Document type: Journal article
• Source: Talanta; v. 246, p. 12-pg., 2022-08-15.

Abstract

Early diagnosis of diseases depends on accessibility and the ability to detect associated biomarkers. Using aptamers and equipping signal transducers with nanomaterials have facilitated, accelerated, and provided sensitive and selective nanoaptasensors. In this study, the first aptasensor to detect heat shock protein 70 kDa (HSP70) has been developed by applying a modified gold electrode (GE) with the lady fern-like gold (LFG) nanostructure. The nanostructure solution contained HAuCl4, H2SO4, and histamine and was electrochemically synthesized on the surface of the GE with an average size of similar to 20 nm. The analysis to find the optimized time for immobilization of aptamer (a single-stranded RNA) as the biorecognition element on the surface of the working electrode was performed using the open-circuit potential (OCP) technique. This aptasensor could detect HSP70 in a linear range from 0.05 to 75 ng mL(-1) with the limit of detection (LOD) similar to 0.02 ng mL(-1). In order to find out about the performance of the designed aptasensor, other analytical analyses for knowing about the figure of metrics were shadowed through reproducibility, stability, regeneration, selectivity, accuracy, and precision experiments. (AU)

• FAPESP's process: 14/50867-3 - INCT 2014: National Institute of Science and Technology in Bioanalysis
• Grantee: Marco Aurelio Zezzi Arruda
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 19/27021-4 - Design and fabrication of electrochemical biosensors for early diagnosis of myocardial infarction and Hepatocellular Carcinoma using microRNAs (miRNAs) primers as biorecognition element and arrays of gold nanostructures
• Grantee: Masoud Negahdary
• Support Opportunities: Scholarships in Brazil - Post-Doctoral