Silver-Coated Silica Nanoparticles Modified with MPS: Potential Antimicrobial Biomaterials Applied in Glaze and Soft Reliner

Author(s)

Rossi, Natalia Rivoli ; Canuto de Menezes, Beatriz Rossi ; Sampaio, Aline da Graca ; da Silva, Diego Morais ; Koga-Ito, Cristiane Yumi ; Thim, Gilmar Patrocinio ; de Arruda Paes-Junior, Tarcisio Jose

• Document type: Journal article
• Source: POLYMERS; v. 14, n. 20, p. 19-pg., 2022-10-01.

Abstract

Soft reliner and glaze are materials used over full or partial dental prosthesis to prevent excessive pressure on the supporting tissues. They are also indicated as supportive treatment for dental stomatitis, especially when modified by the addition of medications. The objective of the work was to evaluate the antimicrobial effect of silver-coated silica nanoparticles in a glaze and a soft reliner. The nanoparticles were synthesized, characterized, and tested by minimum inhibitory concentration (MIC) for C. albicans SC5314. Then, the nanoparticles were incorporated to a glaze and a soft reliner, which were called nanocomposites. Then, the nanocomposites were divided into six groups (n = 12): CG: glaze/reliner; CR: reliner; G1: glaze + 1% nanoparticles/reliner; G2: glaze + 2.5% nanoparticles/reliner; R1: reliner + 1%; R2: reliner + 2.5%. The nanocomposites were characterized by a goniometer and by a scanning electron microscope. The antibiofilm test was performed against C. albicans SC5314. According to the MIC results, the non-functionalized nanoparticles reduced fungal growth at 1000 mu g/mL and the functionalized nanoparticles at 2000 mu g/mL. The functionalized nanoparticle had a superior dispersion being selected for the antibiofilm test. There was a reduction of 64% in CFU/specimen count for the glaze, not statistically significant (p = 0.244). For the soft reliner, there was an increase in CFU/specimen with the presence of nanoparticles, still not statistically significant (p = 0.264). In conclusion, it is necessary to conduct new studies to increase the release of silver, thus improving nanoparticles' antifungal potential. (AU)

• FAPESP's process: 20/12507-6 - Development and characterization of wollastonite cement for bone tissue engineering
• Grantee: Gilmar Patrocínio Thim
• Support Opportunities: Regular Research Grants