Cytochrome C with Peroxidase-like Activity Supported on Plasmonic AuNPs: Improved Stability and Enhanced Nanobioplasmonic Catalytic Conversion

Author(s)

da Silva, Rafael T. P. ; de Barros, Heloise. R. R. ; Fernandes, Rafaella. F. F. ; Temperini, Marcia L. A. ; de Torresi, Susana. I. Cordoba I.

• Document type: Journal article
• Source: CHEMCATCHEM; v. N/A, p. 9-pg., 2023-02-01.

Abstract

Metallic nanoparticles have been extensively used to adsorb biomolecules and improve their stability under broader conditions. This study focuses on the interaction of Cytochrome C (CytC) and gold nanoparticles (AuNP), CytC@AuNP. Circular dichroism (CD) and Raman show significant differences in secondary structure, mainly in alpha-helix. CD ramp temperatures revealed enhanced thermal stability of CytC@AuNP, as evidenced by a refolding of the alpha-helix conformation of CytC and a delay in denaturation temperature by approximately 15 degrees C. Catalytic oxidation performed with CytC@AuNP showed a hyperbolic profile up to 104 % higher yields compared to CytC, which exhibited a linear profile and 30 % loss of activity after thermal treatment. The same reaction performed upon plasmon stimulus comparatively increased the catalytic yield of CytC@AuNP in 12 %, although it had no effect on free CytC. This study highlights the advantages of adsorbing biomolecules on nanoparticles and is a glimpse at plasmon-driven nanobiocatalysis. (AU)

• FAPESP's process: 18/25422-9 - Biofilm synthesis based on lichen beta glucans: study of properties and identification of its vibrational signatures
• Grantee: Rafaella Ferreira Fernandes
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 19/09668-0 - Understanding the biophysiochemical interactions between plasmonic nanoparticles and enzymes to obtain bio-nanomaterials with controlled properties
• Grantee: Heloise Ribeiro de Barros
• Support Opportunities: Scholarships abroad - Research Internship - Post-doctor
• FAPESP's process: 18/13492-2 - Synthetic and natural scaffolds applied to regenerative medicine
• Grantee: Luiz Henrique Catalani
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 16/21070-5 - Vibrational spectroscopy with spatial resolution
• Grantee: Mauro Carlos Costa Ribeiro
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 17/20892-4 - Nanobioplasmonic catalysts: a novel approach to controlling the selectivity in light-driven one-pot reactions
• Grantee: Heloise Ribeiro de Barros
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 15/26308-7 - Optimization of the physicochemical properties of nano -structured materials for applications in molecular recognition, catalysis and energy conversion/storage
• Grantee: Roberto Manuel Torresi
• Support Opportunities: Research Projects - Thematic Grants