Measuring concentrations of a dye in the hemolymph of a marine amphipod: Development of a protocol for exposure assessment

Author(s)

dos Santos, Amanda ; Vannuci-Silva, Monizze ; Vendemiatti, Josiane A. de Souza ; Artal, Mariana Coletty ; da Silva, Bianca Ferreira ; Boldrin Zanoni, Maria Valnice ; Umbuzeiro, Gisela de Aragao

• Document type: Journal article
• Source: Marine Pollution Bulletin; v. 175, p. 5-pg., 2022-02-04.

Abstract

The increasing pollution of aquatic environments due to old and emerging contaminants requires the develop-ment of integrative methods for exposure assessment. Internal concentrations are a reliable way to estimate total exposure of contaminants originated from different routes (water, sediment, and food). We developed a protocol to evaluate the concentration of a dye, C.I. Disperse Red 1, in the hemolymph of Parhyale hawaiensis, a marine amphipod. LOD and LOQ were satisfactory to detect the dye in all hemolymph samples. The concentration detected in the hemolymph varied related to exposure time and dye concentration (0.003 to 0.086 mu g mL(-1)). Polynomial regression model was the best fit. The protocol was reliable to detect and quantify dye exposure in marine amphipods and can be considered for future assessments of estuarine and marine regions under the in-fluence of dye processing plants. The method possibly can be easily adapted to other amphipods and other azo dyes. (AU)

• FAPESP's process: 13/26301-7 - Development and application of method for silver determination in the hemolymph of amphipods exposed to nanomaterials
• Grantee: Monizze Vannuci e Silva
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 14/08829-7 - Developing ecotoxicity biomarkers in the marine amphipod Parhyale hawaiensis and testing toxicity of manufactured nanomaterials
• Grantee: Mariana Coletty Artal
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 08/10449-7 - Assessment of occurrence, toxicity/genotoxicity and degradation processes of dyes in effluents and surface water
• Grantee: Maria Valnice Boldrin
• Support Opportunities: Research Projects - Thematic Grants