Porous layer open tubular nano liquid chromatography directly coupled to electron ionization mass spectrometry

Author(s)

Medina, Deyber Arley Vargas ; Burato, Juliana Soares da Silva ; Borsatto, Joao Victor Basolli ; Lancas, Fernando Mauro

• Document type: Journal article
• Source: Journal of Chromatography A; v. 1674, p. 9-pg., 2022-05-16.

Abstract

A 25 mu m i.d x 1.2 m length PS-DVB porous layer open tubular column (PLOT) was prepared and assessed in the configuration of a nano liquid chromatography coupled to an electron ionization mass spectrometry system (OT-nanoLC-EI-Ms), via the direct insertion of the column outlet into the ionization source. The developed system's operational parameters were comprehensively studied, and the setup performance was investigated employing both unidimensional and column switching configurations. As a result, the OT-nanoLC-EI-MS system demonstrated competitive applicability in separating non-amenable ESI compounds, such as polyaromatic hydrocarbons (PAHs) and non-amenable GC compounds such as thermolabile pesticides. Furthermore, with excellent chromatographic performance, the PLOT columns can work under more compatible EI-detection conditions - such as the elution with 100% organic solvent. For example, PAHs retention factors ranged between 1.5 and 2.2 for 100% MeCN mobile phase, and more than 33,0 0 0 plates per meter for naphthalene at 50 nL/min flow rate. In analyzing thermolabile pesticides, the column switching PLOT-nanoLC-EI-MS system provided LODs of 25 mu g/L, demonstrating suitable intra e interday reproducibility (% RSD < 13%, n = 3), and possibilities the direct injection of raw samples with suitable robustness.(c) 2022 Elsevier B.V. All rights reserved. (AU)

• FAPESP's process: 17/02147-0 - Single drop chromatography and its coupling to mass spectrometry: instrumental strategies, development of materials, automation and analytical applications
• Grantee: Fernando Mauro Lanças
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 19/22724-7 - Novel instrumental approaches for automated and miniaturized analytical methods for the detection of organic compounds by liquid chromatography coupled to mass spectrometry
• Grantee: Deyber Arley Vargas Medina
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 14/07347-9 - Development of novel materials and devices for miniaturized sample preparation techniques in the area of food safety
• Grantee: Fernando Mauro Lanças
• Support Opportunities: Regular Research Grants
• FAPESP's process: 15/15462-5 - Employment of graphene as sorbent in miniaturized sample preparation techniques.
• Grantee: Bruno Henrique Fumes
• Support Opportunities: Scholarships in Brazil - Doctorate