| Full text | |
| Author(s): |
Ferigolo, Leticia F.
;
Vicente, Mateus H.
;
Nogueira, Fabio T. S.
Total Authors: 3
|
| Document type: | Journal article |
| Source: | PLASMID; v. 121, p. 8-pg., 2022-04-12. |
| Abstract | |
Gateway system is one of the most known cloning systems, which makes it compatible with several expression vectors, including those used for Yeast Two-Hybrid (Y2H) and Bimolecular Fluorescence Complementation (BiFC) assays. However, this system is laborious and expensive due to its two-step cloning. In this research, we developed a new cloning strategy named Brick into the Gateway (BiG). This approach uses GoldenBraid/Gate assemblies to create a DNA fragment of interest flanked by attL sites, which can be directly recombined into Gateway destination vectors. BiG method showed a high recombination efficiency and ensured the correct reading frame, which was successfully tested in Y2H and BiFC assays. BiG has proven to be a rapid, low-cost, reusable, and directional cloning method which allows the merged use of systems. (AU) | |
| FAPESP's process: | 19/20157-8 - Evaluation of possible interactions between photoperiodic, age and gibereline flowering pathway with SP/SFT module and its impacts on tomato architecture (Solanum lycopersicum L.) |
| Grantee: | Mateus Henrique Vicente |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 18/17441-3 - Molecular control of shoot architecture: interplay between microRNAs, transcription factors and phytohormones |
| Grantee: | Fabio Tebaldi Silveira Nogueira |
| Support Opportunities: | Research Projects - Thematic Grants |