Enzymatic synthesis of geranyl acetate in batch and fed-batch reactors and evaluation of its larvicidal activity against Rhipicephalus (Boophilus) microplus

Author(s)

Remonatto, Daniela ; Fantatto, Rafaela Regina ; Pietro, Rosemeire Cristina Linhari Rodrigues ; Monti, Rubens ; Oliveira, J. Vladimir ; de Paula, Ariela Veloso ; Bassan, Juliana Cristina

• Document type: Journal article
• Source: Process Biochemistry; v. 120, p. 14-pg., 2022-06-27.

Abstract

Geraniol esters, such as geranyl acetate, are known to have antimicrobial and insect-repellent action, but their acaricidal activity has been little explored. This study aimed to investigate the solvent-free synthesis of geranyl acetate in batch (BSTR) and fed-batch (FBSTR) stirred tank reactors using the immobilized lipases NS 88011 and Novozym 435 and evaluate its larvicidal activity against the cattle tick Rhipicephalus (Boophilus) microplus. A central composite design (CCD) was used to maximize the synthesis of geranyl acetate in a BSTR. The independent variables were temperature reaction (40-60 degrees C), acetic acid/geraniol molar ratio (1:1-1:3), and enzyme loading (1-5% w/w in relation to the substrates). Novozym 435 yielded the best results, achieving 99% of geranyl acetate conversion using 1:3 acetic acid/geraniol molar ratio, 60 degrees C and 5% (w/w) of enzyme loading after 1 h of reaction in a BSTR. In the FBSTR system, a high content of geranyl acetate was obtained (about 80%) in 4 h of reaction using a 1:1 acetic acid/geraniol molar ratio, 60 degrees C and 5% (w/w) Novozym 435. Geranyl acetate showed potential as an acaricide, with an LC90 of 6.25 mg/mL and 100% mortality at a concentration of 12.5 mg/mL. (AU)

• FAPESP's process: 20/09592-1 - Nanoencapsulated bioactive esters: enzymatic synthesis and study of potential antimicrobial
• Grantee: Ariela Veloso de Paula
• Support Opportunities: Regular Research Grants