| Full text | |
| Author(s): |
Niciura, Simone Cristina Meo
;
Benavides, Magda Vieira
;
Okino, Cintia Hiromi
;
Ibelli, Adriana Mercia Guaratini
;
Minho, Alessandro Pelegrine
;
Esteves, Sergio Novita
;
Chagas, Ana Carolina de Souza
Total Authors: 7
|
| Document type: | Journal article |
| Source: | PATHOGENS; v. 11, n. 8, p. 12-pg., 2022-08-01. |
| Abstract | |
Among the gastrointestinal nematodes affecting sheep, Haemonchus contortus is the most prevalent and virulent, resulting in health problems and production losses. Therefore, selecting sheep resistant to H. contortus is a suitable and sustainable strategy for controlling endoparasites in flocks. Here, 287 lambs of the native Brazilian Morada Nova hair sheep breed were subjected to two consecutive artificial infections with H. contortus and assessed for fecal egg count (FEC), packed cell volume (PCV), and live weight (LW). Forty-four animals ranked as having extreme resistance phenotypes were genotyped using the Illumina OvineSNP50v3 chip. A case-control genome-wide association study (GWAS) detected 37 significant (p < 0.001) markers in 12 ovine chromosomes in regions harboring quantitative trait loci (QTL) for FEC, Trichostrongylus spp. adults and larvae, weight, and fat; and candidate genes for immune responses, mucins, hematological parameters, homeostasis, and growth. Four single-nucleotide polymorphisms (SNP; OAR1_rs427671974, OAR2_rs419988472, OAR5_rs424070217, and OAR17_rs401006318) genotyped by qPCR followed by high-resolution melting (HRM) were associated with FEC and LW. Therefore, molecular markers detected by GWAS for H. contortus resistance in Morada Nova sheep may support animal selection programs aimed at controlling gastrointestinal nematode infections in flocks. Furthermore, genotyping of candidate genes using HRM qPCR may provide a rapid and efficient tool for animal identification. (AU) | |
| FAPESP's process: | 19/02967-2 - Development of methods to study Haemonchus contortus and anthelmintic resistance to monepantel |
| Grantee: | Simone Cristina Méo Niciura |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 17/01626-1 - Genetic and immune responses characterization associated with the phenotype of parasite resistance in a sheep flock of Morada Nova |
| Grantee: | Ana Carolina de Souza Chagas |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 19/02929-3 - Target selective treatment based on liveweight gain as nematode control strategy in Morada Nova lamb |
| Grantee: | Ana Carolina de Souza Chagas |
| Support Opportunities: | Regular Research Grants |