| Full text | |
| Author(s): |
Crulhas, Bruno P.
;
Basso, Caroline R.
;
Castro, Gustavo R.
;
Pedrosa, Valber A.
Total Authors: 4
|
| Document type: | Journal article |
| Source: | Current Medicinal Chemistry; v. 29, n. 37, p. 8-pg., 2022-01-01. |
| Abstract | |
Background: Prostate cancer cells have very high PCA3 messenger RNA levels, which turns them into one of the new biomarkers for prostate cancer prognosis and diagnosis. Objective: Our goal here is to develop a new aptasensor to detect PCA3 release by the cancer cell. Methods: DNA hairpin containing PCA3 aptamer was thiolated, conjugated to methylene blue (MB) redox probe, and immobilized on gold electrode through self-assembly to detect label-free cancer cells. Results: Our data have evidenced stable and sensitive sensors presenting a wide linear detection range (0-150ng/mL). In addition, monitoring PCA3 released by different types of prostate cells can provide in-depth knowledge about prostate cancer dynamics; therefore, it is a powerful platform for earlier clinical diagnostic. The released PCA3 can vary depending on the type of adopted prostate cells. Conclusion: PCA3 release was monitored in a group of cells for 2 h; it showed significantly higher expression in both LNCaP and PC-3 cells. This strategy provides a unique and simple methodology to achieve more sensitive and specific PCA3 detection; thus, it emerged as a promising tool for early cost-effective diagnosis (AU) | |
| FAPESP's process: | 19/13411-5 - Development of microfluidic device for liquid biopsy of circulating tumor cells |
| Grantee: | Valber de Albuquerque Pedrosa |
| Support Opportunities: | Regular Research Grants |