Shotgun proteomics of Nanicao and Prata bananas reveals changes that may account for their different resistance to low temperature

Author(s)

Facundo, Heliofabia Virginia de Vasconcelos ; Schmitz, Gabriela Justamante Handel ; Cataldi, Thais Regiani ; Labate, Carlos Alberto ; Nascimento, Joao Roberto Oliveira do ; Lajolo, Franco Maria

• Document type: Journal article
• Source: Scientia Horticulturae; v. 306, p. 11-pg., 2022-08-25.

Abstract

Low-temperature storage has been widely used to extend the post-harvest life of many fruits. However, tolerance to cold is largely variable even among different fruit cultivars. In this regard, the banana cultivars Nanicao and Prata display different responses to low-temperature storage. Prata is less affected by cold storage, while the synthesis of volatiles is drastically reduced in Nanicao bananas. Therefore, this study aimed to investigate the effect of low temperature storage at the proteome level using a shotgun proteomics approach to identify a large number of proteins in these banana varieties. Seventy-four differentially expressed proteins were identified in cv. Prata and 43 in cv. Nanicao. Principal component analysis indicated that samples from control and cold storage groups could be differentiated based on their proteome. Overall, low temperatures decreased proteins related to carbohydrate metabolism and energy, defense, and transport in Nanicao bananas. Contrastingly, low temperatures induced the accumulation of proteins for carbohydrate metabolism and energy, transport, and defense in Prata bananas. Therefore, the observed changes in the proteome during cold acclimation and ripening in Nanicao and Prata bananas can explain, at least in part, the differences in low-temperature tolerance between these cultivars. (AU)

• FAPESP's process: 13/07914-8 - FoRC - Food Research Center
• Grantee: Bernadette Dora Gombossy de Melo Franco
• Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC
• FAPESP's process: 09/14958-6 - Low temperature storage of different banana cultivars: the effect in attributes of quality
• Grantee: Heliofábia Virgínia de Vasconcelos Facundo
• Support Opportunities: Scholarships in Brazil - Doctorate