Pristina longiseta reproduction test: chronic exposure to environmental contaminants

Author(s)

Cunha de Souza, Tallyson Tavares ; Castro, Gleyson Borges ; Bernegossi, Aline Christine ; Felipe, Mayara Caroline ; Pinheiro, Fernanda Rodrigues ; Colombo-Corbi, Vanessa ; Girolli, Douglas Aparecido ; Gorni, Guilherme Rossi ; Corbi, Juliano Jose

• Document type: Journal article
• Source: Environmental Science and Pollution Research; v. N/A, p. 11-pg., 2022-11-03.

Abstract

Aquatic worms are considered a suitable group to evaluate the effects of contaminants on the environment, although one of the main challenges is to use the species of local occurrence. Recently, Pristina longiseta was suggested to be used in acute bioassays. In this context, this study aimed to establish a chronic exposure for ecotoxicological bioassays using the cosmopolitan species of occurrence in Brazilian freshwater P. longiseta. Firstly, we tested three exposure times (4, 7, and 10 days) under the presence or absence of aeration for reproduction outputs. After determining the best configuration (7 days without aeration), we assessed the effects of the chronic exposures using the standardized reference substance potassium chloride (KCl), the antibiotic sulfamethoxazole (SMX), the flame retardant tetrabromobisphenol A (TBBPA), and the sugarcane vinasse. Our results showed suitability for applying the chronic exposure using P. longiseta and indicated the sensitivity of the offspring to KCl (EC50-7d = 0.51 g/L). Sulfamethoxazole and TBBPA caused a significant decrease in the offspring of P. longiseta (EC50-7d = 59.9 mu g/L and < 62.5 mu g/L, respectively). Sugarcane vinasse showed high toxicity for the species, and 4.26% of vinasse was calculated as EC50-7d. Therefore, the described protocol was successfully applied as an ecotoxicological bioassay to evaluate the effects of environmental contaminants on the reproduction rate of the freshwater worm P. longiseta. (AU)

• FAPESP's process: 20/11042-0 - Antibiotics in synthetic and hospital wastewater, raw and treated on anaerobic reactors: toxic effects on freshwater invertebrates
• Grantee: Gleyson Borges Castro
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 20/09912-6 - Ativa-ETE: evaluation of innovative technologies for the removal of nitrogen and micropollutants in wastewater treatment plants (WWTP)
• Grantee: José Alberto Domingues Rodrigues
• Support Opportunities: Research Grants - Research Partnership for Technological Innovation - PITE