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Light Boosts the Activity of Novel LPMO from Aspergillus fumigatus Leading to Oxidative Cleavage of Cellulose and Hemicellulose

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Author(s):
Velasco, Josman ; Sepulchro, Ana Gabriela V. ; Higasi, Paula M. R. ; Pellegrini, Vanessa O. A. ; Cannella, David ; de Oliveira, Leandro Cristante ; Polikarpov, Igor ; Segato, Fernando
Total Authors: 8
Document type: Journal article
Source: ACS SUSTAINABLE CHEMISTRY & ENGINEERING; v. 10, n. 50, p. 16-pg., 2022-12-08.
Abstract

Lytic polysaccharide monooxygenases (LPMOs) are copper-dependent enzymes that potentiate the deconstruction of polysaccharides through an oxidative mechanism. The oxidative catalysis of LPMOs is dependent on a reductant to promote the divalent copper ion in a metalloenzyme to its monovalent state. The reported molecules that can activate LPMOs are organic compounds of low molecular weight, enzymes, lignin and lignin-derived compounds, and, recently, photosynthetic pigments. This work reports the functional characterization and the computational modeling of the threedimensional structure of a novel LPMO from Aspergillus fumigatus var. niveus (AfAA9C). AfAA9C shows the ability to oxidize glucose residues in the cellulose chain at C1-and C4-carbon, being the first reported LPMO from A. fumigatus active on xyloglucan and capable of being activated by light. The evaluation of electron donors coupled to chlorophyllin + light photosystems allowed to elucidate the existence of a collaborative effect between a chemical reducing agent and light-induced electron transfer systems promoting changes in LPMO activity, which is reducing agent-type-dependent. The results suggest that the preference of AfAA9C for a specific reducing agent is altered when the compound is associated with the photosystem due to H2O2 generation. These findings are of general importance for the utilization of LPMOs in reactions applying photobiocatalysis and in sustainable industrial processes such as biomass depolymerization. (AU)

FAPESP's process: 19/22284-7 - Biochemical, functional characterization and application of polysaccharide lytic monoxygenases identified in transcriptome and secretoma studies of filamentous fungi cultivated in sugarcane bagasse
Grantee:Fernando Segato
Support Opportunities: Program for Research on Bioenergy (BIOEN) - Regular Program Grants
FAPESP's process: 18/22300-0 - Synergistic activities to boost enzymatic lignocellulose oxidation using light and LPMOs
Grantee:Igor Polikarpov
Support Opportunities: Regular Research Grants
FAPESP's process: 19/01165-0 - New recombinant PMOS from Aspergillus fumigatus: biochemical characterization and its effect on saccharification of sugarcane bagasse with different pre-treatments
Grantee:Josman Andrey Velasco Mendoza
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 21/06679-1 - Enzymatic oxidation of sugarcane bagasse 2: exploring the interaction between LPMOs and its redox partner CDHs aiming for the development of more efficient enzymes for insertion into an engineered cell factory
Grantee:Fernando Segato
Support Opportunities: Program for Research on Bioenergy (BIOEN) - Young Investigators Grants - Phase 2
FAPESP's process: 14/18714-2 - Enzymatic oxidation of sugarcane bagasse: discovery, characterization and new application of oxidative enzymes active in carbohydrates, applied to the enhancement of a fungal cell factory
Grantee:Fernando Segato
Support Opportunities: Program for Research on Bioenergy (BIOEN) - Young Investigators Grants
FAPESP's process: 15/13684-0 - Structural and functional studies of enzymes that participate in complex carbohydrates synthesis and degradation
Grantee:Igor Polikarpov
Support Opportunities: Research Projects - Thematic Grants