Histological, Immunohistochemical and Antioxidant Analysis of Skin Wound Healing Influenced by the Topical Application of Brazilian Red Propolis

Author(s)

Conceicao, Mariana ; Sergio Gushiken, Lucas Fernando ; Aldana-Mejia, Jennyfer Andrea ; Tanimoto, Matheus Hikaru ; de Sa Ferreira, Marcos Vital ; Miranda Alves, Andreia Cristina ; Miyashita, Marina Naomi ; Bastos, Jairo Kenupp ; Beserra, Fernando Pereira ; Pellizzon, Claudia Helena

• Document type: Journal article
• Source: ANTIOXIDANTS; v. 11, n. 11, p. 20-pg., 2022-11-01.

Abstract

Skin wound healing is a complex process that requires the mutual work of cellular and molecular agents to promote tissue restoration. In order to improve such a process, especially in cases of impaired healing (e.g., diabetic ulcer, chronic wounds), there is a search for substances with healing properties and low toxicity: two features that some natural products-such as the bee product named propolis-exhibit. Propolis is a resinous substance obtained from plant resins and exudates with antioxidant, anti-inflammatory, and antitumoral activities, among other biological ones. Based on the previously reported healing actions of different types of propolis, the Brazilian red propolis (BRP) was tested for this matter. A skin wound excision model in male Wistar rats was performed using two topical formulations with 1% red propolis as treatments: hydroalcoholic extract and Paste. Macroscopical, histological and immunohistochemical analysis were performed, revealing that red propolis enhanced wound contraction, epithelialization, reduced crust formation, and modulated the distribution of healing associated factors, mainly collagen I, collagen III, MMP-9, TGF-beta 3 and VEGF. Biochemical analysis with the antioxidants SOD, MPO, GSH and GR showed that propolis acts similarly to the positive control, collagenase, increasing these molecules' activity. These results suggest that BRP promotes enhanced wound healing by modulating growth factors and antioxidant molecules related to cutaneous wound healing. (AU)

• FAPESP's process: 21/01258-8 - Characterization of fibroblasts and endothelial cells activity on wound healing in rats treated topically with red Brazilian propolis
• Grantee: Mariana Conceição
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 17/04138-8 - Attainment of chemical, analytical, biological, pharmacological and technological studies to fill the gaps on the development of Brazilian propolis sector
• Grantee: Jairo Kenupp Bastos
• Support Opportunities: Research Projects - Thematic Grants