Microbiological Properties and Cytotoxicity of PNVCL Hydrogels Containing Flavonoids as Intracanal Medication for Endodontic Therapy

Author(s)

Braga, Gabriela Pacheco de Almeida ; Caiaffa, Karina Sampaio ; Pereira, Jesse Augusto ; dos Santos, Vanessa Rodrigues ; Souza, Amanda Caselato Andolfatto ; Ribeiro, Lucas da Silva ; Camargo, Emerson Rodrigues ; Prakki, Anuradha ; Duque, Cristiane

• Document type: Journal article
• Source: JOURNAL OF FUNCTIONAL BIOMATERIALS; v. 13, n. 4, p. 16-pg., 2022-12-01.

Abstract

This study aimed to evaluate the cytotoxicity and microbiological properties of poly (N-vinylcaprolactam)-PNVCL hydrogels containing flavonoids as intracanal medication for endodontic therapy. Antimicrobial activity of ampelopsin (AMP), isoquercitrin and rutin was determined against Enterococcus faecalis, Actinomyces israelii, Lactobacillus casei, Streptococcus mutans, and Fusobacterium nucleatum by the microdilution method. After synthesis and characterization by rheology, PNVCL hydrogels were loaded with AMP and controls calcium hydroxide (CH) and chlorhexidine (CHX), and determined the compounds release profile. PNVCL+AMP, PNVCL+CH, PNVCL+CHX were evaluated on multi-species biofilms and analyzed by Scanning Electron Microscopy (SEM) and Confocal Laser Scanning Microscopy (CLSM). Cytotoxicity was determined after fibroblasts exposure to serial dilutions of AMP and PNVCL hydrogel extracts. AMP was effective against all of the bacteria tested, especially against S. mutans, A. israelli and F. nucleatum. SEM and CLSM analysis showed that PNVCL + AMP caused a significant decrease and disorganization of multi-species biofilms and reduction of intracanal viable cells, superior to the other groups. AMP affected fibroblast viability at concentrations above 0.125 mg/mL, and extracts of PNVCL+AMP showed low cytotoxicity. In conclusion, PNVCL containing AMP demonstrated cytocompatibility and potent effect against multi-species biofilms and could be potential intracanal medication for endodontic purposes. (AU)

• FAPESP's process: 17/10940-1 - Flavonoids combinations in thermosensitive hidrogels as proposals of endodontic medications for young permanent teeth
• Grantee: Cristiane Duque
• Support Opportunities: Regular Research Grants
• FAPESP's process: 18/12871-0 - Modification of the temperature transition profile of thermosensitive nanocomposites
• Grantee: Lucas da Silva Ribeiro
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)