Hydrodynamic Cavitation-Assisted Oxidative Pretreatment and Sequential Production of Ethanol and Xylitol as Innovative Approaches for Sugarcane Bagasse Biorefineries

Author(s)

Prado, C. A. ; Cunha, M. L. S. ; Teran-Hilares, R. ; Arruda, G. L. ; Antunes, F. A. F. ; Pereira, B. ; da Silva, S. S. ; Santos, J. C.

• Document type: Journal article
• Source: BioEnergy Research; v. N/A, p. 13-pg., 2023-01-14.

Abstract

In the present work, a new alternative of hydrodynamic cavitation-assisted pretreatment associated with an advanced oxidative process was proposed, together with a new approach to obtain bioproducts in sequential fermentations. Enzymatic hydrolysate of sugarcane bagasse (SCB) was fermented to ethanol by Saccharomyces cerevisiae. After distillation of this alcohol, the obtained vinasse was used to produce xylitol by Candida tropicalis. Influent variables in pretreatment were evaluated by performing experiments according to a statistical design, and, at optimum conditions (ozone flowrate of 10 mg/min, and H2O2 concentration of 0.61%), about 84% and 78% of glucan and xylan hydrolysis yields were obtained in enzymatic hydrolysis, respectively. In the sequential fermentations for ethanol and xylitol production, yield values of 0.41 g/g and 0.55 g/g, respectively, were obtained, with corresponding volumetric productivities of 8.33 g/Lh and 0.64 g/Lh, respectively. The proposed strategy was shown as a promising approach for biorefineries, considering the mild conditions of pretreatment and the possibility of high ethanol production using S. cerevisiae in a fermentation process similar to that one already available in sucro-alcoholic sector, followed by xylitol production in vinasse-based medium. (AU)

• FAPESP's process: 16/10636-8 - From the cell factory to the Biodiesel-Bioethanol integrated biorefinery: a systems approach applied to complex problems in micro and macroscales
• Grantee: Roberto de Campos Giordano
• Support Opportunities: Program for Research on Bioenergy (BIOEN) - Thematic Grants
• FAPESP's process: 20/12059-3 - Alternative technologies for ethanol and pullulan production in sugarcane bagasse biorefineries using hydrodynamic cavitation reactors and systems for simultaneous hydrolysis and fermentation
• Grantee: Júlio César dos Santos
• Support Opportunities: Program for Research on Bioenergy (BIOEN) - Regular Program Grants
• FAPESP's process: 20/16638-8 - Evaluation of 2G ethanol production from sugarcane bagasse pretreated by hydrodynamic cavitation-assisted oxidative process: pretreatment conditions and utilization of simultaneous hydrolysis and fermentation reactors with interconnected columns system
• Grantee: Maria Laura Silva Cunha
• Support Opportunities: Scholarships in Brazil - Scientific Initiation