RP-HPLC simultaneous quantification of rutin, avobenzone, and octyl methoxycinnamate in the presence of hydroxypropyl ?-cyclodextrin (HP?CD) and sulfobutyl ether ?-cyclodextrin (SBE?CD)

Author(s)

Martins, Tercio Elyan Azevedo ; Pinto, Claudineia Aparecida Sales de Oliveira ; Oliveira, Andressa Costa de ; Lima, Fabiana Vieira ; Velasco, Maria Valeria Robles ; Rodrigues, Leticia Norma Carpentieri ; Baby, Andre Rolim

• Document type: Journal article
• Source: Brazilian Journal of Pharmaceutical Sciences; v. 58, p. 16-pg., 2022-01-01.

Abstract

Development and validation of a simple and fast method of high-performance liquid chromatography with diode array detection (HPLC-DAD) for the simultaneously analysis of rutin, avobenzone, and octyl p-methoxycinnamate is presented. These substances were separated using a Kromasil C18 (250x4.6 mm, 5 mu m) column, methanol: water (88:12 v/v) as the mobile phase, and a flow rate of 0.8 mL min-1. The experiment was performed at room temperature and elution was under isocratic conditions. Quantification was performed by external calibration at the wavelength of 325 nm. The validated parameters included linearity, selectivity, precision (repeatability), intermediate precision, accuracy, limit of detection, limit of quantification and robustness. The results of validation were statistically treated using the Action Stat version 3.5.152.34. The selectivity was also evaluated in the presence of two cyclodextrins (2-hydroxypropyl-beta-cyclodextrin and beta-cyclodextrin sulfobutyl ether sodium). The absence of parallelism between the curves of octyl p-methoxycinnamate in the absence and presence of the beta-cyclodextrin sulfobutyl ether sodium in the mobile phase revealed interference from this matrix, thereby indicating the necessity of validating the method in the presence of this, and other matrices. The proposed method was selective, linear, precise, accurate, and robust for the simultaneous determination of rutin, avobenzone, and octylp-methoxycinnamate. (AU)

• FAPESP's process: 19/16169-0 - Ex vivo and in vivo establishment of new chemopreventive properties of UV filters
• Grantee: André Rolim Baby
• Support Opportunities: Regular Research Grants