Development of a portable lens-free holographic microscope to imaging cell cultures

Author(s)

D'Almeida, Camila de Paula ; Feitosa, Patrick Oliveira ; de Oliveira, Natalia Portes ; Pratavieira, Sebastiao ; IEEE

• Document type: Journal article
• Source: 2021 SBFOTON INTERNATIONAL OPTICS AND PHOTONICS CONFERENCE (SBFOTON IOPC); v. N/A, p. 4-pg., 2021-01-01.

Abstract

Microscopes are essential imaging tools for accessing micro scale information in several contexts, including routine visual analysis for cell culture. As an alternative to traditional optical microscopic instrumentation, lens-free microscopy is an emerging modality, which is efficient to provide portable apparatus that provides images with decoupling resolution from the field of view. This paper presents compact and easy-to-use imaging equipment based on optical lens-free holographic microscopy, which performs amplitude and phase images from a sequence of captured images at different distances from the sample, resulting in images with almost 30 square millimeters and 4 micrometers of resolution. (AU)

• FAPESP's process: 14/50857-8 - National Institute in Basic Optics and Applied to Life Sciences
• Grantee: Vanderlei Salvador Bagnato
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 13/07276-1 - CEPOF - Optics and Photonic Research Center
• Grantee: Vanderlei Salvador Bagnato
• Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC