| Full text | |
| Author(s): Show less - |
Roque-Borda, Cesar Augusto
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Souza Saraiva, Mauro de Mesquita
;
Monte, Daniel F. M.
;
Rodrigues Alves, Lucas Bocchini
;
de Almeida, Adriana Maria
;
Ferreira, Taisa Santiago
;
de Lima, Tulio Spina
;
Pereira Benevides, Valdinete
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Cabrera, Julia Memrava
;
Claire, Sunil
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Meneguin, Andreia Bagliotti
;
Chorilli, Marlus
;
Pavan, Fernando Rogerio
;
Berchieri Junior, Angelo
;
Vicente, Eduardo Festozo
Total Authors: 15
|
| Document type: | Journal article |
| Source: | ACS INFECTIOUS DISEASES; v. 8, n. 3, p. 10-pg., 2022-03-11. |
| Abstract | |
Salmonella enterica subsp. enterica serovar Enteritidis (S. Enteritidis) in poultry is most often transmitted by the fecal-oral route, which can be attributed to high population density. Upon encountering the innate immune response in a host, the pathogen triggers a stress response and virulence factors to help it survive in the host. The aim of this study was to evaluate the effect of hypromellose acetate/succinate (HPMCAS)-coated alginate microparticles containing the Ctx(Ile(21))-Ha antimicrobial peptide (AMP) on both intestinal colonization and systemic infection of laying hens challenged with S. Enteritidis. The applied AMP microsystem reduced the bacterial load of S. Enteritidis in the liver, with a statistical significance between groups A (control, no Ctx(Ile(21))-Ha peptide) and B (2.5 mg of Ctx(Ile(21))-Ha/kg) at 2 days postinfection (dpi), potentially indicating the effectiveness of Ctx(Ile(21))-Ha in the first stage of infection by S. Enteritidis. In addition, the results showed a significant decrease in the S. Enteritidis counts in the spleen and cecal content at 5 dpi; remarkably, no S. Enteritidis counts were observed in livers at 5, 7, and 14 dpi, regardless of the Ctx(Ile(21))-Ha dosage (p-value <0.0001). Using the Chi-square test, the effect of AMP microparticles on S. Enteritidis fecal excretion was also evaluated, and a significantly lower bacterial excretion was observed over 21 days in groups B and C, in comparison with the untreated control (p-value <0.05). In summary, the use of HPMCAS-Ctx(Ile(21))-Ha peptide microcapsules in laying hens drastically reduced the systemic infection of S. Enteritidis, mainly in the liver, indicating a potential for application as a feed additive against this pathogen. (AU) | |
| FAPESP's process: | 20/13497-4 - Search for the mechanism of action and therapeutic effect of new classes of drugs against Mycobacterium tuberculosis |
| Grantee: | Fernando Rogério Pavan |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 18/25707-3 - Application of the antimicrobial peptide Ctx(Ile21)-Ha and its conjugates in different formulations as a nutritional additive in laying hens |
| Grantee: | Cesar Augusto Roque Borda |
| Support Opportunities: | Scholarships in Brazil - Master |
| FAPESP's process: | 20/16573-3 - In vitro and in vivo studies of antimicrobial peptide B1CTcu5 analogs encapsulated in colon-specific microparticles against Mycobacterium tuberculosis |
| Grantee: | Cesar Augusto Roque Borda |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 16/00446-7 - Application of antimicrobial peptides as new additives in animal nutrition |
| Grantee: | Eduardo Festozo Vicente |
| Support Opportunities: | Research Grants - Young Investigators Grants |