Using Chitosan-Coated Polymeric Nanoparticles-Thermosensitive Hydrogels in association with Limonene as Skin Drug Delivery Strategy

Author(s)

Campos, Estefania V. R. ; Proenca, Patricia L. F. ; da Costa, Tais G. ; de Lima, Renata ; Fraceto, Leonardo F. ; de Araujo, Daniele R.

• Document type: Journal article
• Source: BIOMED RESEARCH INTERNATIONAL; v. 2022, p. 18-pg., 2022-04-07.

Abstract

Topical delivery of local anesthetics (LAs) is commonly used to decrease painful sensations, block pain throughout procedures, and alleviate pain after surgery. Dermal and/or transdermal delivery of LAs has other advantages, such as sustained drug delivery and decreased systemic adverse effects. This study reports the development of poly(D,L-lactide-co-glycolide) (PLGA) nanoparticles coated with chitosan for the sustained release and topicality of benzocaine (BZC) and topical delivery. BZC PLGA nanoparticles or nonencapsulated drugs were further incorporated into Poloxamer hydrogels (Pluronic (TM) F-127). The nanoparticles showed a mean diameter of 380 +/- 4 nm, positive zeta potential after coating with chitosan (23.3 +/- 1.7 mV), and high encapsulation efficiency (96.7 +/- 0.02%). Cellular viability greater than 70% for both fibroblasts and keratinocytes was observed after treatment with nanoparticles, which is in accordance with the preconized guidelines for biomedical devices and delivery systems. Both the nanoparticles and hydrogels were able to modulate BZC delivery and increase drug permeation when compared to the nonencapsulated drug. Furthermore, the incorporation of limonene into hydrogels containing BZC-loaded nanoparticles increased the BZC permeation rates. Non-Newtonian and pseudoplastic behaviors were observed for all hydrogel nanoformulations with or without nanoparticles. These results demonstrate that the hydrogel-nanoparticle hybrids could be a promising delivery system for prolonged local anesthetic therapy. (AU)

• FAPESP's process: 19/05100-0 - Evaluation of the biological activity of topical formulations of budesonide in inflammatory skin models
• Grantee: Estefânia Vangelie Ramos Campos
• Support Opportunities: Scholarships abroad - Research Internship - Post-doctor
• FAPESP's process: 17/24402-1 - pH AND TEMPERATURE SENSITIVE DRUG NANOCARRIERS FOR SKIN APPLICATIONS
• Grantee: Estefânia Vangelie Ramos Campos
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 19/20303-4 - Thermosensitive organogels as strategies for the treatment of inflammatory processes: from supramolecular structure to pharmacological evaluation
• Grantee: Daniele Ribeiro de Araujo
• Support Opportunities: Regular Research Grants