Preparation, characterization and biological evaluation of nitric oxide releasing polymeric biomaterials

• Grant number: 17/05029-8
• Support type: Scholarships in Brazil - Doctorate
• Effective date (Start): June 01, 2017
• Effective date (End): December 31, 2020
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Physical-Chemistry
• Cooperation agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Amedea Barozzi Seabra
• Grantee: Milena Trevisan Pelegrino
• Home Institution: Centro de Ciências Naturais e Humanas (CCNH). Universidade Federal do ABC (UFABC). Ministério da Educação (Brasil). Santo André , SP, Brazil

Abstract

S-nitrosothiols (RSNOs) are an important class of nitric oxide (NO) donors, involved in several biological processes, such as: (i) control of blood vasodilatation, (ii) antioxidant, antitumoral and antimicrobial activities, (iii) promotion and acceleration of wound healing (iv) regeneration of skin, cartilage, mucosa and globe eye tissues. RSNOs act as NO storage in vivo preserving NO physiological activities. Recently, the combination of RSNOs and biomaterials represents a promising strategy to increase the therapeutical efficacy of RSNOs. In this context, the main purpose of this project is the preparation of RSNOs and their incorporation into nanoparticles and biocompatible hydrogels. RSNOs will be incorporated in chitosan nanoparticles, coated with hyaluronic acid, for targeting the NO-releasing nanoparticles to tumour tissues. Chitosan is a biocompatible and biodegradable biopolymer, employed in the synthesis of nanoparticles for controllable and sustainable drug release. The biopolymer hyaluronic acid has been used for coating nanoparticles to tumour targeting purposes. In addition, the project involves the incorporation of either free RSNOs or encapsulated RSNOs into thermosensitive hydrogels composed by Pluronic F-127/chitosan and chitosan based visible light crosslinkable hydrogels for biomedical applications, including skin regeneration. The project involves the preparation and characterization of NO releasing nanoparticles and hydrogels, the evaluation of the NO release profile from the biomaterials, and biological assays to evaluate and investigate the therapeutic actions of the prepared biomaterials. These new materials are expected to have great potential in biomedical applications. (AU)

Scientific publications

(12)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PELEGRINO, MILENA T.; PIERETTI, JOANA C.; NAKAZATO, GERSON; GONCALVES, MARCELLY CHUE; MOREIRA, JOSE CARLOS; SEABRA, AMEDEA B. Chitosan chemically modified to deliver nitric oxide with high antibacterial activity. NITRIC OXIDE-BIOLOGY AND CHEMISTRY, v. 106, p. 24-34, JAN 1 2021. Web of Science Citations: 0.

KOHATSU, MARCIO YUKIHIRO; PELEGRINO, MILENA TREVISAN; MONTEIRO, LUCILENA REBELO; FREIRE, BRUNA MOREIRA; PEREIRA, RODRIGO MENDES; FINCHEIRA, PAOLA; RUBILAR, OLGA; TORTELLA, GONZALO; BATISTA, BRUNO LEMOS; DE JESUS, TATIANE ARAUJO; SEABRA, AMEDEA BAROZZI; LANGE, CAMILA NEVES. Comparison of foliar spray and soil irrigation of biogenic CuO nanoparticles (NPs) on elemental uptake and accumulation in lettuce. Environmental Science and Pollution Research, v. 28, n. 13 JAN 2021. Web of Science Citations: 0.

CAIO-SILVA, WELLINGTON; DA SILVA DIAS, DANIELLE; JUNHO, CAROLINA VICTORIA CRUZ; PANICO, KARINE; NERES-SANTOS, RAQUEL SILVA; PELEGRINO, MILENA TREVISAN; PIERETTI, JOANA CLAUDIO; SEABRA, AMEDEA BAROZZI; DE ANGELIS, KATIA; CARNEIRO-RAMOS, MARCELA SORELLI. Characterization of the Oxidative Stress in Renal Ischemia/Reperfusion-Induced Cardiorenal Syndrome Type 3. BIOMED RESEARCH INTERNATIONAL, v. 2020, OCT 9 2020. Web of Science Citations: 0.

PISSOLATO, MARIA DOLORES; SILVEIRA, NEIDIQUELE MARIA; PRATAVIERA, PAULA JOYCE CARRENHO; MACHADO, EDUARDO CARUSO; SEABRA, AMEDEA BAROZZI; PELEGRINO, MILENA TREVISAN; SODEK, LADASLAV; RIBEIRO, RAFAEL V. Enhanced Nitric Oxide Synthesis Through Nitrate Supply Improves Drought Tolerance of Sugarcane Plants. FRONTIERS IN PLANT SCIENCE, v. 11, JUN 30 2020. Web of Science Citations: 0.

PELEGRINO, MILENA T.; PAGANOTTI, ANDRE; SEABRA, AMEDEA B.; WELLER, RICHARD B. Photochemistry of nitric oxide andS-nitrosothiols in human skin. Histochemistry and Cell Biology, v. 153, n. 6, p. 431-441, JUN 2020. Web of Science Citations: 1.

PIERETTI, JOANA C.; PELEGRINO, MILENA T.; NASCIMENTO, MONICA H. M.; TORTELLA, GONZALO R.; RUBILAR, OLGA; SEABRA, AMEDEA B. Small molecules for great solutions: Can nitric oxide-releasing nanomaterials overcome drug resistance in chemotherapy?. Biochemical Pharmacology, v. 176, n. SI JUN 2020. Web of Science Citations: 0.

PELEGRINO, MILENA TREVISAN; KOHATSU, MARCIO YUKIHIRO; SEABRA, AMEDEA BAROZZI; MONTEIRO, LUCILENA REBELO; GOMES, DIEGO GENUARIO; OLIVEIRA, HALLEY CAIXETA; ROLIM, WALLACE ROSADO; DE JESUS, TATIANE ARAUJO; BATISTA, BRUNO LEMOS; LANGE, CAMILA NEVES. Effects of copper oxide nanoparticles on growth of lettuce (Lactuca sativa L.) seedlings and possible implications of nitric oxide in their antioxidative defense. ENVIRONMENTAL MONITORING AND ASSESSMENT, v. 192, n. 4 MAR 12 2020. Web of Science Citations: 0.

PELEGRINO, MILENA T.; WELLER, RICHARD B.; PAGANOTTI, ANDRE; SEABRA, AMEDEA B. Delivering nitric oxide into human skin from encapsulated S-nitrosoglutathione under UV light: An in vitro and ex vivo study. NITRIC OXIDE-BIOLOGY AND CHEMISTRY, v. 94, p. 108-113, JAN 1 2020. Web of Science Citations: 0.

NASCIMENTO, MONICA H. M.; PELEGRINO, MILENA T.; PIERETTI, JOANA C.; SEABRAL, AMEDEA B. How can nitric oxide help osteogenesis?. AIMS MOLECULAR SCIENCE, v. 7, n. 1, p. 29-48, 2020. Web of Science Citations: 0.

LOPES-OLIVEIRA, PATRICIA JULIANA; GOMES, DIEGO GENUARIO; PELEGRINO, MILENA TREVISAN; BIANCHINI, EDMILSON; PIMENTA, JOSE ANTONIO; STOLF-MOREIRA, RENATA; SEABRA, AMEDEA BAROZZI; OLIVEIRA, HALLEY CAIXETA. Effects of nitric oxide-releasing nanoparticles on neotropical tree seedlings submitted to acclimation under full sun in the nursery. SCIENTIFIC REPORTS, v. 9, NOV 22 2019. Web of Science Citations: 0.

PELEGRINO, MILENA T.; LIMA, BRUNA DE ARAUJO; DO NASCIMENTO, MONICA H. M.; LOMBELLO, CHRISTIANE B.; BROCCHI, MARCELO; SEABRA, AMEDEA B. Biocompatible and Antibacterial Nitric Oxide-Releasing Pluronic F-127/Chitosan Hydrogel for Topical Applications. POLYMERS, v. 10, n. 4 APR 2018. Web of Science Citations: 7.

PELEGRINO, MILENA T.; SEABRA, AMEDEA B. Chitosan-Based Nanomaterials for Skin Regeneration. AIMS MEDICAL SCIENCE, v. 4, n. 3, p. 352-381, 2017. Web of Science Citations: 3.