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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Characterization of the Oxidative Stress in Renal Ischemia/Reperfusion-Induced Cardiorenal Syndrome Type 3

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Caio-Silva, Wellington [1] ; da Silva Dias, Danielle [2] ; Junho, Carolina Victoria Cruz [1] ; Panico, Karine [1] ; Neres-Santos, Raquel Silva [1] ; Pelegrino, Milena Trevisan [1] ; Pieretti, Joana Claudio [1] ; Seabra, Amedea Barozzi [1] ; De Angelis, Katia [2] ; Carneiro-Ramos, Marcela Sorelli [1]
Total Authors: 10
[1] Fed Univ ABC, Ctr Nat & Human Sci CCNH, Ave Estados 5001, BR-09210170 Santo Andre, SP - Brazil
[2] Univ Fed Sao Paulo, Dept Physiol, Santo Andre 862, BR-04023062 Sao Paulo, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Web of Science Citations: 0

In kidney disease (KD), several factors released into the bloodstream can induce a series of changes in the heart, leading to a wide variety of clinical situations called cardiorenal syndrome (CRS). Reactive oxygen species (ROS) play an important role in the signaling and progression of systemic inflammatory conditions, as observed in KD. The aim of the present study was to characterize the redox balance in renal ischemia/reperfusion-induced cardiac remodeling. C57BL/6 male mice were subjected to occlusion of the left renal pedicle, unilateral, for 60 min, followed by reperfusion for 8 and 15 days, respectively. The following redox balance components were evaluated: catalase (CAT), superoxide dismutase (SOD), total antioxidant capacity (FRAP), NADPH oxidase (NOX), nitric oxide synthase (NOS), hydrogen peroxide (H2O2), and the tissue bioavailability of nitric oxide (NO) such as S-nitrosothiol (RSNO) and nitrite (NO2-). The results indicated a process of renoprotection in both kidneys, indicated by the reduction of cellular damage and some oxidant agents. We also observed an increase in the activity of antioxidant enzymes, such as SOD, and an increase in NO bioavailability. In the heart, we noticed an increase in the activity of NOX and NOS, together with increased cell damage on day 8, followed by a reduction in protein damage on day 15. The present study concludes that the kidneys and heart undergo distinct processes of damage and repair at the analyzed times, since the heart is a secondary target of ischemic kidney injury. These results are important for a better understanding of the cellular mechanisms involved in CRS. (AU)

FAPESP's process: 18/02832-7 - Hybrid magnetic and antibacterial nanoparticles: synthesis, characterization and applications
Grantee:Joana Claudio Pieretti
Support type: Scholarships in Brazil - Master
FAPESP's process: 18/08194-2 - Essential oil amended with metal nanoparticles functionalized with nitric oxide as a strategy to control of plant pathogens in the agriculture
Grantee:Amedea Barozzi Seabra
Support type: Regular Research Grants
FAPESP's process: 08/10175-4 - Impact of inflammatory response induced by acute renal ischemia in cardiac tissue
Grantee:Marcela Sorelli Carneiro Ramos
Support type: Research Grants - Young Investigators Grants
FAPESP's process: 17/05029-8 - Preparation, characterization and biological evaluation of nitric oxide releasing polymeric biomaterials
Grantee:Milena Trevisan Pelegrino
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 19/11077-0 - Cardiac alterations induced by renal inflammation models: participation of the Klotho / FGF-23 axis
Grantee:Marcela Sorelli Carneiro Ramos
Support type: Regular Research Grants