Characterization of the Oxidative Stress in Renal Ischemia/Reperfusion-Induced Cardiorenal Syndrome Type 3

Author(s)

Caio-Silva, Wellington ^[1] ; da Silva Dias, Danielle ^[2] ; Junho, Carolina Victoria Cruz ^[1] ; Panico, Karine ^[1] ; Neres-Santos, Raquel Silva ^[1] ; Pelegrino, Milena Trevisan ^[1] ; Pieretti, Joana Claudio ^[1] ; Seabra, Amedea Barozzi ^[1] ; De Angelis, Katia ^[2] ; Carneiro-Ramos, Marcela Sorelli ^[1]

Affiliation

^[1] Fed Univ ABC, Ctr Nat & Human Sci CCNH, Ave Estados 5001, BR-09210170 Santo Andre, SP - Brazil

^[2] Univ Fed Sao Paulo, Dept Physiol, Santo Andre 862, BR-04023062 Sao Paulo, SP - Brazil

• Document type: Journal article
• Source: BIOMED RESEARCH INTERNATIONAL; v. 2020, OCT 9 2020.
• Web of Science Citations: 0

Abstract

In kidney disease (KD), several factors released into the bloodstream can induce a series of changes in the heart, leading to a wide variety of clinical situations called cardiorenal syndrome (CRS). Reactive oxygen species (ROS) play an important role in the signaling and progression of systemic inflammatory conditions, as observed in KD. The aim of the present study was to characterize the redox balance in renal ischemia/reperfusion-induced cardiac remodeling. C57BL/6 male mice were subjected to occlusion of the left renal pedicle, unilateral, for 60 min, followed by reperfusion for 8 and 15 days, respectively. The following redox balance components were evaluated: catalase (CAT), superoxide dismutase (SOD), total antioxidant capacity (FRAP), NADPH oxidase (NOX), nitric oxide synthase (NOS), hydrogen peroxide (H2O2), and the tissue bioavailability of nitric oxide (NO) such as S-nitrosothiol (RSNO) and nitrite (NO2-). The results indicated a process of renoprotection in both kidneys, indicated by the reduction of cellular damage and some oxidant agents. We also observed an increase in the activity of antioxidant enzymes, such as SOD, and an increase in NO bioavailability. In the heart, we noticed an increase in the activity of NOX and NOS, together with increased cell damage on day 8, followed by a reduction in protein damage on day 15. The present study concludes that the kidneys and heart undergo distinct processes of damage and repair at the analyzed times, since the heart is a secondary target of ischemic kidney injury. These results are important for a better understanding of the cellular mechanisms involved in CRS. (AU)

• FAPESP's process: 18/02832-7 - Hybrid magnetic and antibacterial nanoparticles: synthesis, characterization and applications
• Grantee: Joana Claudio Pieretti
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 18/08194-2 - Essential oil amended with metal nanoparticles functionalized with nitric oxide as a strategy to control plant pathogens in the agriculture
• Grantee: Amedea Barozzi Seabra
• Support Opportunities: Regular Research Grants
• FAPESP's process: 08/10175-4 - Impact of inflammatory response induced by acute renal ischemia in cardiac tissue
• Grantee: Marcela Sorelli Carneiro Ramos
• Support Opportunities: Research Grants - Young Investigators Grants
• FAPESP's process: 17/05029-8 - Preparation, characterization and biological evaluation of nitric oxide releasing polymeric biomaterials
• Grantee: Milena Trevisan Pelegrino
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 19/11077-0 - Cardiac alterations induced by renal inflammation models: participation of the Klotho/FGF-23 axis
• Grantee: Marcela Sorelli Carneiro Ramos
• Support Opportunities: Regular Research Grants