Electrospun PHBV nanofiber containing Tea Tree Oil: physicochemical and antimicrobial activity

Author(s)

Verônica Ribeiro dos Santos ^[1] ; Samara Domingues Vera ^[2] ; Gabrielle Lupeti de Cena ^[3] ; Adrielle de Paula Silva ^[4] ; Ana Paula Lemes ^[5] ; Kátia da Conceição ^[6] ; Dayane Batista Tada ^[7] ; Alexandre Luiz Souto Borges ^[8] ; Eliandra de Sousa Trichês ^[9]

Affiliation

^[1] Universidade Federal de São Paulo. Instituto de Ciência e Tecnologia. Laboratório de Biocerâmicas - Brasil

^[2] Universidade Federal de São Paulo. Instituto de Ciência e Tecnologia. Laboratório de Biocerâmicas - Brasil

^[3] Universidade Federal de São Paulo. Instituto de Ciência e Tecnologia. Laboratório de Bioquímica de Pepitídeos - Brasil

^[4] Universidade Federal de São Paulo. Instituto de Ciência e Tecnologia. Laboratório de Nanomateriais e Nanotoxicologia - Brasil

^[5] Universidade Federal de São Paulo. Instituto de Ciência e Tecnologia. Laboratório de Tecnologia em Polímeros e Biopolímeros - Brasil

^[6] Universidade Federal de São Paulo. Instituto de Ciência e Tecnologia. Laboratório de Bioquímica de Pepitídeos - Brasil

^[7] Universidade Federal de São Paulo. Instituto de Ciência e Tecnologia. Laboratório de Nanomateriais e Nanotoxicologia - Brasil

^[8] Universidade Estadual Paulista. Instituto de Ciência e Tecnologia. Departamento de Materiais Dentários e Prostodontia - Brasil

^[9] Universidade Federal de São Paulo. Instituto de Ciência e Tecnologia. Laboratório de Biocerâmicas - Brasil

• Document type: Journal article
• Source: POLIMEROS-CIENCIA E TECNOLOGIA; v. 33, n. 1 2023-04-14.

Abstract

Abstract Aiming to produce an antimicrobial dressing for wound healing applications, in this work Tea Tree oil (TTO) was incorporated into PHBV nanofibers by absorption. It was observed increase in the nanofiber diameter due to 5% TTO absorption efficiency, which also led to a 54% decrease in the contact angle. The releasing assay indicates a 6.8% oil release in the first 24 h – being probably the oil deposited at the polymer surface – followed by a minimal release at 48 h. The set of antimicrobial assays performed suggests the incorporation of TTO optimized the antimicrobial activity of the polymer for E. coli and C. albicans, while against S. aureus no significant difference was observed. The MTT assay showed no cytotoxicity of PHBV, but the incubation of L929 fibroblast cells with PHBV-TTO reduced cell viability. Overall, the PHBV nanofibers containing TTO present great potential as an antimicrobial dressing. (AU)

• FAPESP's process: 19/10877-3 - PHBV nanofibers containing sol-gel derived bioactive borate glasses doped with therapeutic ions for tissue regeneration
• Grantee: Verônica Ribeiro dos Santos
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 19/19594-4 - 3D pritting of bioactive and resorbable scaffolds by robocasting: synthesis, processing and antibacterial activity
• Grantee: Eliandra de Sousa Trichês
• Support Opportunities: Regular Research Grants
• FAPESP's process: 20/04455-6 - PHBV nanofibers containing Melaleuca oil by electrospinning: a new generation of antimicrobian biomaterials
• Grantee: Samara Domingues Vera
• Support Opportunities: Scholarships in Brazil - Scientific Initiation