Vaterite microparticle-loaded methylene blue for photodynamic activity in macrophages infected with Leishmania braziliensis

Author(s)

Marmo, Vitor Luca Moura ; Ambrosio, Jessica A. R. ; Goncalves, Erika Peterson ; Raniero, Leandro Jose ; Beltrame Junior, Milton ; Pinto, Juliana G. ; Ferreira-Strixino, Juliana ; Simioni, Andreza R.

• Document type: Journal article
• Source: PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES; v. 22, n. 8, p. 13-pg., 2023-04-28.

Abstract

Calcium carbonate (CaCO3) exhibits a variety of crystalline phases, including the anhydrous crystalline polymorphs calcite, aragonite, and vaterite. Developing porous calcium carbonate microparticles in the vaterite phase for the encapsulation of methylene blue (MB) as a photosensitizer (PS) for use in photodynamic therapy (PDT) was the goal of this investigation. Using an adsorption approach, the PS was integrated into the CaCO3 microparticles. The vaterite microparticles were characterized by scanning electron microscopy (SEM) and steady-state techniques. The trypan blue exclusion method was used to measure the biological activity of macrophages infected with Leishmania braziliensis in vitro. The vaterite microparticles produced are highly porous, non-aggregated, and uniform in size. After encapsulation, the MB-loaded microparticles kept their photophysical characteristics. The carriers that were captured allowed for dye localization inside the cells. The results obtained in this study indicated that the MB-loaded vaterite microparticles show promising photodynamic activity in macrophages infected with Leishmania braziliensis. (AU)

• FAPESP's process: 18/18531-6 - Polyelectrolyte microcapsules as a model of controlled release system for photosensitive drugs
• Grantee: Andreza Ribeiro Simioni
• Support Opportunities: Regular Research Grants