Photobiomodulation and vitamin B treatment alleviate both thermal and mechanical orofacial pain in rats

Author(s)

Marques, Daniel Pereira ; Chacur, Marucia ; Martins, Daniel Oliveira

• Document type: Journal article
• Source: PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES; v. 22, n. 10, p. 13-pg., 2023-06-20.

Abstract

Purpose The present study investigates the efficacy of Photobiomodulation (PBM) and Vitamin B Complex (VBC) to relieve pain, both in separately and combined (PBM and VBC). Methods Rats with chronic constriction injury of the right infraorbital nerve (CCI-IoN) or Sham surgery were used. PBM was administered at a wavelength of 904 nm and energy density of 6.23 J/cm(2) and VBC (containing B1, B6 and B12) subcutaneously, both separately and combined. Behavioral tests were performed to assess mechanical and thermal hypersensitivity before and after CCI and after PBM, VBC, or PBM + VBC. The expression of inflammatory proteins in the trigeminal ganglion and the immunohistochemical alterations of Periaqueductal Gray (PAG) astrocytes and microglia were examined following CCI and treatments. Results All testeds treatments reversed the painful behavior. The decrease in pain was accompanied by a decrease of Glial Fibrillary Acidic Protein (GFAP), a specific astrocytic marker, and Ionized calcium-binding adaptor molecule 1 (Iba-1), a marker of microglia, and decreased expression of Transient Receptor Potential Vanilloid 1 (TRPV1), Substance P, and Calcitonin Gene-Related Peptide (CGRP) induced by CCI-IoN in PAG and Trigeminal ganglion. Furthermore, both treatments showed a higher expression of Cannabinoid-type 1 (CB1) receptor in the trigeminal ganglion compared to CCI-IoN rats. Our results show that no difference was observed between groups. Conclusion We showed that PBM or VBC regulates neuroinflammation and reduces inflammatory protein expression. However, the combination of PBM and VBC did not enhance the effectiveness of both therapies alone. [GRAPHICS] (AU)

• FAPESP's process: 21/02897-4 - Effect of photobiomodulation treatment on mitochondrial dynamics: analysis of the dorsal root ganglion in a type 1 Diabetes mellitus model
• Grantee: Marucia Chacur
• Support Opportunities: Regular Research Grants
• FAPESP's process: 17/05218-5 - Effect of photobiostimulation on different peripheral neuropathies in rats.
• Grantee: Marucia Chacur
• Support Opportunities: Regular Research Grants
• FAPESP's process: 14/24533-0 - Photodynamic therapy in trigeminal neuralgia
• Grantee: Marucia Chacur
• Support Opportunities: Regular Research Grants
• FAPESP's process: 15/24256-0 - Study of the structures involved in orofacial pain and perspectives of combined treatment of low level laser and B vitamins in a model of trigeminal neuralgia
• Grantee: Daniel de Oliveira Martins
• Support Opportunities: Scholarships in Brazil - Post-Doctoral