Phomactinine, the First Nitrogen-Bearing Phomactin, Produced by Biatriospora sp. CBMAI 1333

Author(s):Show less -

Oliveira, Leandro da Silva ; Crnkovic, Camila M. M. ; de Amorim, Marcelo R. R. ; Navarro-Vazquez, Armando ; Paz, Tiago A. A. ; Freire, Vitor F. F. ; Takaki, Mirelle ; Venancio, Tiago ; Ferreira, Antonio G. G. ; Saito, Renata de Freitas ; Chammas, Roger ; Berlinck, Roberto G. S.

• Document type: Journal article
• Source: Journal of Natural Products; v. N/A, p. 8-pg., 2023-07-25.

Abstract

Metabolomics analyses and improvement of growth conditionswereapplied toward diversification of phomactin terpenoids by the fungus Biatriospora sp. CBMAI 1333. Visualization of molecularnetworking results on Gephi assisted the observation of phomactindiversification and guided the isolation of new phomactin variantsby applying a modified version of chemometrics based on a fractionalfactorial design. Consequentially, the first nitrogen-bearing phomactin,phomactinine (1), with a new rearranged carbon skeleton,was isolated and identified. The strategy combining metabolomics andchemometrics can be extended to include bioassay potency, structurenovelty, and metabolic diversification connected or not to genomicanalyses. (AU)

• FAPESP's process: 13/50228-8 - Biodiversity components, and their metabolic characters, of Brazilian Islands: an integrated approach
• Grantee: Roberto Gomes de Souza Berlinck
• Support Opportunities: BIOTA-FAPESP Program - Thematic Grants
• FAPESP's process: 19/00447-1 - Improving and diversifying the production and investigating the biosynthesis of phomactins by the cultured fungus Biatriospora sp. CBMAI 1333
• Grantee: Camila Manoel Crnkovic
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 20/01229-5 - Bioactive metabolites of bacterial flora microorganisms from non-filtering marine animals
• Grantee: Marcelo Rodrigues de Amorim
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 19/17721-9 - The role of Chemistry in holobiont adaptation
• Grantee: Roberto Gomes de Souza Berlinck
• Support Opportunities: Research Projects - Thematic Grants