Reduced Graphene Oxide Electrolyte-Gated Transistor Immunosensor with Highly Selective Multiparametric Detection of Anti-Drug Antibodies

Author(s):Show less -

Sensi, Matteo ; de Oliveira, Rafael Furlan ; Berto, Marcello ; Palmieri, Marina ; Ruini, Emilio ; Livio, Pietro Antonio ; Conti, Andrea ; Pinti, Marcello ; Salvarani, Carlo ; Cossarizza, Andrea ; Cabot, Joan M. ; Ricart, Jordi ; Casalini, Stefano ; Gonzalez-Garcia, Maria Begona ; Fanjul-Bolado, Pablo ; Bortolotti, Carlo Augusto ; Samori, Paolo ; Biscarini, Fabio

• Document type: Journal article
• Source: Advanced Materials; v. 35, n. 36, p. 9-pg., 2023-07-28.

Abstract

The advent of immunotherapies with biological drugs has revolutionized the treatment of cancers and auto-immune diseases. However, in some patients, the production of anti-drug antibodies (ADAs) hampers the drug efficacy. The concentration of ADAs is typically in the range of 1-10 pm; hence their immunodetection is challenging. ADAs toward Infliximab (IFX), a drug used to treat rheumatoid arthritis and other auto-immune diseases, are focussed. An ambipolar electrolyte-gated transistor (EGT) immunosensor is reported based on a reduced graphene oxide (rGO) channel and IFX bound to the gate electrode as the specific probe. The rGO-EGTs are easy to fabricate and exhibit low voltage operations (& LE; 0.3 V), a robust response within 15 min, and ultra-high sensitivity (10 am limit of detection). A multiparametric analysis of the whole rGO-EGT transfer curves based on the type-I generalized extreme value distribution is proposed. It is demonstrated that it allows to selectively quantify ADAs also in the co-presence of its antagonist tumor necrosis factor alpha (TNF-& alpha;), the natural circulating target of IFX. (AU)

• FAPESP's process: 21/06238-5 - Functionalized solution-processed 2D materials: development of prototyping electric sensors and biosensors
• Grantee: Rafael Furlan de Oliveira
• Support Opportunities: Research Grants - Young Investigators Grants