Mutanase from Trichoderma harzianum inductively Produced by Mutan: Short-Term Treatment to Degrade Mature Streptococcus mutans Biofilm

Author(s)

Bem, Jessica Silva Peixoto ; Lacerda, Nayanna Gomes Silva ; Polizello, Ana Cristina Morseli ; Cabral, Hamilton ; da Rosa-Garzon, Nathalia Gonsales ; Aires, Carolina Patricia

• Document type: Journal article
• Source: Current Microbiology; v. 80, n. 9, p. 10-pg., 2023-09-01.

Abstract

This study aimed to evaluate the disruptive effect of fungal mutanase against cariogenic biofilm after short-term treatment. For that, mature Streptococcus mutans biofilms (n = 9) were exposed to active or inactivated enzymes produced by Trichoderma harzianum for 1 min, two times per day. Biofilms were analyzed by amount of matrix water-insoluble polysaccharides, bacterial viability, acidogenicity, and morphology by scanning electron microscopy (SEM). The group treated with active enzymes (AE) had a significantly lower amount of insoluble polysaccharides (893.30 & PLUSMN; 293.69) when compared to the negative control group (NaCl, 2192.59 & PLUSMN; 361.96), yet no significant difference was found when comparing to the positive control group (CHX, 436.82 & PLUSMN; 151.07). Also, there was no significant effect on bacteria metabolism and viability (P-value < 0.05). Data generated by the quantitative analysis were confirmed through scanning electron microscopy images. Thus, fungal mutanase degraded the biofilm after a short-term treatment without interfering with bacterial viability and metabolism. Such findings offer insight to the development of routine oral care products containing this input. (AU)

• FAPESP's process: 19/19162-7 - The challenge of biofilm control in patients using palatal breaker: effect of enzymes produced by the fungus Trichoderma harzianum
• Grantee: Nayanna Gomes Silva Lacerda
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 20/07315-0 - Mango processing by-products for cariogenic biofilm control: in vitro study
• Grantee: Jéssica Silva Peixoto Bem
• Support Opportunities: Scholarships in Brazil - Master