Gene expression and biochemical profiling in the mitigation of heat stress in common bean using Bacillus subtilis

Author(s)

Lima, B. C. ; Cruz, T. R. ; Ribas, A. F. ; Santos, T. B. ; Cacefo, V. ; Araujo, F. F.

• Document type: Journal article
• Source: Biologia Plantarum; v. 67, p. 11-pg., 2023-01-01.

Abstract

The present work aimed to evaluate the effect of heat stress on common bean (Phaseolus vulgaris L.) genotypes during the reproductive phase as a function of the inoculation of plants with Bacillus subtilis. The treatments were established by inoculating two strains of B. subtilis (AP-3 and AP-12) and a control. The plants were subjected to heat stress when they reached the reproductive stage, with an increase in temperature to 28/33 & DEG;C. The duration of the stress period was ten days. Flowering, biochemical, and gene expression evaluations were performed. There was the interaction of B. subtilis AP-3 with the bean cultivar IAC-Imperador, reducing flower abortion, promoting the formation of new flower buds, and increasing the content of proline and guaiacol peroxidase activity in plant tissues. However, there was a reduction of transcription of genes encoding the 1-carboxylic acid-1aminocyclopropane oxidase and ethylene response factors and an increase of the & UDelta;1-pyrroline-5-carboxylate synthetase1 gene. These results suggest that B. subtilis may modulate some metabolic pathways in response to high-temperature stress during the reproductive phase of the common bean. This also confirms that Bacillus strains represent a useful option to moderate abiotic stresses. (AU)

• FAPESP's process: 17/16202-2 - Bacillus subtilis on attenuation of heat stress and modulation of ethylene in bean
• Grantee: Fabio Fernando de Araujo
• Support Opportunities: Regular Research Grants
• FAPESP's process: 21/10823-0 - Microbial community and its relationship with the suppression of nematodes in a crop-livestock integration system
• Grantee: Fabio Fernando de Araujo
• Support Opportunities: Regular Research Grants