Antibacterial, antibiofilm and anticaries effect of BioXtra® mouthrinse for head and neck cancer (HNC) patients under a microcosm biofilm model

Author(s)

da Silva, Natara Dias Gomes ; da Silva Santos, Paulo Sergio ; Magalhaes, Ana Carolina ; Buzalaf, Marilia Afonso Rabelo

• Document type: Journal article
• Source: RADIOTHERAPY AND ONCOLOGY; v. 187, p. 8-pg., 2023-10-01.

Abstract

Background and purpose: Considering the lack of studies investigating salivary substitutes to control postradiation caries for patients with head and neck cancer (HNC), this study aimed to evaluate the antibacterial, antibiofilm, and anticaries effects of BioXtra & REG; on the microcosm biofilm formed on different enamel types (non-irradiated and irradiated) and from distinct saliva sources (control and HNC patients). Materials and methods: Non-irradiated and irradiated enamel specimens were treated with BioXtra & REG;, phosphate-buffered-saline (PBS; negative control), or 0.12% chlorhexidine (CHX; positive control) for 1 min. Biofilm was produced from human saliva (healthy participants with normal salivary flow for the control group or irradiated HNC patients with hyposalivation for the HNC group), mixed with McBain saliva, under 0.2% sucrose exposure, daily submitted to the treatments (1 min), for 5 days. Bacterial metabolic activity, biofilm viability, CFU counting, and enamel demineralization were determined.Results: BioXtra & REG; significantly reduced the bacterial metabolic activity for both enamel types and the inoculum sources, being more effective for the irradiated enamel or for the saliva from the control group. Similarly, BioXtra & REG; significantly reduced the biofilm viability, the CFU for total microorganisms, mutans streptococci, and lactobacilli, and was able to significantly reduce the mineral loss and the lesion depth compared to PBS. CHX was an effective treatment to significantly reduce all parameters, performing better than BioXtra & REG; and reinforcing its reliable efficiency as a positive control.Conclusion: Regardless of the enamel type and the inoculum source, BioXtra & REG; presented antibacterial, antibiofilm, and anticaries effects under this experimental model, which should be confirmed in further clinical studies. (AU)

• FAPESP's process: 18/12041-7 - Safety and efficacy for the prevention of dental erosion of a new cystatin derived from sugarcane (CaneCPI-5): in vitro, in situ and in vivo studies
• Grantee: Marília Afonso Rabelo Buzalaf
• Support Opportunities: Regular Research Grants