Characterization of microbial ecology, Listeria monocytogenes, and Salmonella sp. on equipment and utensil surfaces in Brazilian poultry, pork, and industries

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Tadielo, Leonardo Ereno ; dos Santos, Emanoelli Aparecida Rodrigues ; Possebon, Fabio Sossai ; Schmiedt, Jhennifer Arruda ; Juliano, Lara Cristina Bastos ; Cerqueira-Cezar, Camila Koutsodontis ; de Oliveira, Janaina Prieto ; Sampaio, Aryele Nunes da Cruz Encide ; Melo, Patricia Regina Lopes ; Caron, Evelyn Fernanda Flores ; Pinto, Jose Paes de Almeida Nogueira ; Bersot, Luciano dos Santos ; Pereira, Juliano Goncalves

• Document type: Journal article
• Source: Food Research International; v. 173, p. 12-pg., 2023-11-01.

Abstract

This study aimed to evaluate the level of counting by indicator microorganisms, identify the microbial ecology, detect Listeria monocytogenes and Salmonella sp., and determine the presence of virulence genes and biofilm formation. A total of 480 samples were collected from the surfaces of the equipment and utensils using sterile swabs for the detection of L. monocytogenes and Salmonella sp. and counting mesophilic aerobes, Enterobacteriaceae, Escherichia coli, and Pseudomonas sp. The microbial ecology was evaluated by sequencing the 16S rRNA gene. Genes for virulence and biofilm formation were analyzed and adhesion capacity was evaluated for L. monocytogenes and Salmonella sp. The mesophilic aerobe count was the highest in the dairy processing facility, followed by the pork and poultry slaughterhouses. L. monocytogenes was detected in all facilities, with the highest detection in the pork slaughterhouse, followed by the poultry and dairy facilities. Salmonella sp. was only detected in the dairy. Isolates of L. monocytogenes and Salmonella sp. showed poor adhesion to polystyrene surfaces, virulence genes, and biofilm formation. The frequent contaminants in the slaughterhouses were Pseudomonas, Acinetobacter, and Aeromonas in poultry, Acinetobacter, Pseudomonas, and Brevundimonas in pork, and Pseudomonas, Kocuria, and Staphylococcus in dairy. Our results provide useful information to understand the microbiological risks associated with contamination. (AU)

• FAPESP's process: 20/01529-9 - Evaluation of microbial ecology on the presence of Listeria monocytogenes and Salmonella sp. in food processing industries, the formation of multispecies biofilms and control methods
• Grantee: Leonardo Ereno Tadielo
• Support Opportunities: Scholarships in Brazil - Doctorate