In vitro anti-tumoral activity of two versatile cationic porphyrins on melanoma cells

Author(s)

Castro, Kelly A. D. F. ; Biazzotto, Juliana Cristina ; Tome, Joao P. C. ; da Silva, Roberto S. ; Lourenco, Leandro M. O.

• Document type: Journal article
• Source: JOURNAL OF PORPHYRINS AND PHTHALOCYANINES; v. 27, n. 1, p. 7-pg., 2023-01-01.

Abstract

Porphyrin (Por) dyes are considered photoactive entities with potential properties to be applied as photosensitizers (PS) in cancer Photodynamic Therapy (PDT). The use of suitable units, like pyridinium ones, is an important strategy to add peripheral and non-peripheral positive charges in the Por structure, and in that way develop effective cationic PSs for melanoma treatments. In this context, free-base porphyrins bearing thiopyridinium (1) or methoxypyridinium (2) units were studied on melanoma cells, and their PDT effectiveness was studied and compared. The different charge positions of the cationic peripheral units on the Por macrocycle contribute differently to their PDT behavior. The obtained results demonstrate high in vitro PDT efficacy for both PSs. For the highest PS concentration tested (20 mu M) the photocytotoxicity reaches the detection limit of the MTT assay upon 201 seconds of blue light irradiation (lambda = 405 20 nm) at an irradiance of 24.9 mW/cm(2), which corresponds to a light dose of 5 J/cm(2). Interestingly, under the same experimental conditions, cationic Por 1 shows very interesting PDT results at lower concentrations of 1 to 10 mu M. (AU)

• FAPESP's process: 22/15336-3 - Ruthenium complexes and treatment based on electronic excitation: a mechanistic approach focused on cytotoxic assessment by light irradiation, X-rays and ultrasound in 2D and 3D cancer cell lines
• Grantee: Roberto Santana da Silva
• Support Opportunities: Regular Research Grants
• FAPESP's process: 19/19448-8 - Effect of photobiomodulation as synergistic addendum on photocytotoxicity of ruthenium-phthalocyanine compounds as nitric oxide and singlet oxygen producers: in vitro biochemical evaluation in two- and three-dimensional (3D) tumor cell lines
• Grantee: Roberto Santana da Silva
• Support Opportunities: Regular Research Grants