Abstract
The antimicrobial peptides (AMPs) are defense molecules found in different organisms. But, besides its antimicrobial activity, different papers have shown other potentials of these peptides, among then, the antitumoral ability. Our group showed the cytotoxic activity of some PAMs with b-haipin structure (protegrin, tachyplesin, polyphemusin and gomesin) and the action mechanisms that led to the cell death in myeloid eritroleukemic lineages and other kinds of cell as melanoma, neuroblastoma and Chinese hamster ovary cell. We described that those AMPs cause direct membrane destructuring with high concentrations, but different cell events and death with lower doses, varying the AMPs used, although they share great homology between their aminoacid sequence and structure. The present work aims continuing the studies of the AMPs (protegrin, tachyplesin, polyphemusin and gomesin) antileukemic activity, been made: 1) Citotoxic effects comparison between normal and leukemics cells (HL-60, K562 e KG-1), evaluating the molecular and biochemical death events. 2) Differentiation capacity evaluation from AMPs in leukemic stem cells found in leukemic lineages (HL-60, K562 e KG-1). 3) Furthermore, based in our previous studies, in which we have seen morphological differences in cell, we intend to connect this change in cell with the cellular death effects, using advanced computational image techniques and multiparametric analysis, by flow cytometry and fluorescence conventional and confocal microscopy in HELA adhenocarcinoma cells. All the links between the obtained data will be made using computational/mathematic tools.
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