Gomesin acts in the immune system and promotes myeloid differentiation and monocyte/macrophage activation in mouse

Author(s)

Buri, Marcus V. ; Dias, Carol C. ; Barbosa, Christiano M. V. ; Nogueira-Pedro, Amanda ; Ribeiro-Filho, Antonio C. ; Miranda, Antonio ; Paredes-Gamero, Edgar J.

• Document type: Journal article
• Source: Peptides; v. 85, p. 41-45, NOV 2016.
• Web of Science Citations: 3

Abstract

Due to the cytotoxic effect of antimicrobial peptides (AMP) against several microorganism and tumor cells has been proposed their association with the immune system. However, just a few reports have shown this relationship. In this study, mice were treated with gomesin, a beta-hairpin AMP that exhibit high cytotoxicity against bacterial and tumor cells. Different effects in the immune system were observed, such as, decrease of CD3(+) in T lymphocytes (Control: 17.7 +/- 1.4%; Gomesin: 7.67 +/- 11.2%) and in hematopoietic progenitors and increase of hematopoietic stem cell (Control: 0.046 +/- 0.004%; Gomesin: 0.067 +/- 10.003%), B220(+) B lymphocytes (Control: 38.63 +/- 1.5%; Gomesin: 47.83 +/- 0.48%), and Mac-1(+)F4/80(+) macrophages (Control: 11.76 +/- 3.4%; Gomesin: 27.13 +/- 4.0%). Additionally, macrophage increase was accompanied by an increase of macrophage phagocytosis (Control 20.85 +/- 1.53; Gomesin 31.32 +/- 1 Geometric mean), interleukin 6 (Control: 47.24 +/- 1.9 ng/mL; Gomesin: 138.68 +/- 33.68 ng/mL) and monocyte chemoattractant protein-1 (Control: 0.872 +/- 0.093 ng/mL; Gomesin: 1.83 +/- 0.067 ng/mL). Thus, this report showed immunomodulatory activity of gomesin in the immune system of mice. (C) 2016 Elsevier Inc. All rights reserved. (AU)

• FAPESP's process: 13/09068-7 - Action of hematopoietic modulators in leukemic stem cells
• Grantee: Edgar Julian Paredes-Gamero
• Support type: Regular Research Grants
• FAPESP's process: 12/20989-4 - Molecular mechanisms studies in differentiation and cell death by antimicrobial peptides using leukemic and adenocarcinoma cells
• Grantee: Marcus Vinicius Buri
• Support type: Scholarships in Brazil - Doctorate
• FAPESP's process: 15/22901-5 - Photothermal heterodyne image Microscopy single molecule tracking of antimicrobial peptide
• Grantee: Marcus Vinicius Buri
• Support type: Scholarships abroad - Research Internship - Doctorate
• FAPESP's process: 11/17584-0 - Studies of the structure-activity relationship and mechanism of lytic action of the antimicrobial peptide gomesin
• Grantee: Antonio de Miranda
• Support type: Regular Research Grants