Electrochemical detection of xylobiose in banana biomass using a 3D porous copper oxide foam electrode modified with a molecularly imprinted Poly-L-arginine film

Author(s)

da Silva, Mateus Paula ; Beluomini, Maisa Azevedo ; de Freitas, Carolina ; Brienzo, Michel ; Stradiotto, Nelson Ramos

• Document type: Journal article
• Source: Journal of Food Composition and Analysis; v. 124, p. 10-pg., 2023-12-01.

Abstract

Xylobiose (X2) presents numerous health benefits, including cancer prevention, and can be found in agroindustrial biomass, including bananas. Here, an electrochemical sensor based on molecularly imprinted polyL-arginine film (MIP) and 3D porous copper oxide foam (3DnpCu) for the ultrasensitive detection of X2 is studied. Te MIP/3DnpCu-GCE is prepared by dynamic hydrogen bubble template method, followed by electropolymerization of L-arginine in the presence of X2 as template, followed by electrochemical extraction, resulting in the formation of X2 recognition sites. The sensor was characterized using scanning electron microscopy, X-ray photoelectron spectroscopy, and electrochemical impedance spectroscopy. MIP/3DnpCu-GCE showed a wide linear response in the concentration range of 1.0 x 10-12 to 1.0 x 10-10 mol L-1, limit of detection of 7.7 x 10-13 mol L-1 and sensibility of 1.4 mu A pmol-1 L. The sensor exhibited selective X2 recognition, long-term stability (maintaining 86% of its initial current over 8 days), while inter-electrode repeatability displayed an RSD of 2.4%. The applicability of the MIP/3DnpCu-GCE in real samples is demonstrated by successfully quantifying X2 concentration in banana biomass. Furhermore, the comparison between the data obtained using this method and those found by the HPLC method confirmed the accuracy of the sensor. (AU)

• FAPESP's process: 18/12131-6 - Wireless electrochemical sensors based screen-printed electrodes modified with metallic nanopores for determination of flavonoids in wastes of the citrus industry
• Grantee: Maísa Azevedo Beluomini
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 21/02550-4 - Electrochemical sensors based on electrodes modified with copper nanopores and molecularly imprinted polymers for determination of xylobiosis in banana peel and pulp
• Grantee: Mateus Paula da Silva
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 17/22401-8 - Fruit-refinery: obtention processes, characterization methods and generation of products derived from residues of the fruitculture
• Grantee: Nelson Ramos Stradiotto
• Support Opportunities: Research Projects - Thematic Grants