Endophytic, extremophilic and entomophilic fungi strains biodegrade anthracene showing potential for bioremediation

Author(s)

Bonatti, Erika ; dos Santos, Alef ; Birolli, Willian Garcia ; Rodrigues-Filho, Edson

• Document type: Journal article
• Source: WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY; v. 39, n. 6, p. 18-pg., 2023-06-01.

Abstract

Anthropogenic activities have been increasing Polycyclic Aromatic Hydrocarbons (PAHs) release, promoting an urgent need for decontamination methods. Therefore, anthracene biodegradation by endophytic, extremophilic, and entomophilic fungi was studied. Moreover, a salting-out extraction methodology with the renewable solvent ethanol and the innocuous salt K2HPO4 was employed. Nine of the ten employed strains biodegraded anthracene in liquid medium (19-56% biodegradation) after 14 days at 30 degrees C, 130 rpm, and 100 mg L-1. The most efficient strain Didymellaceae sp. LaBioMMi 155, an entomophilic strain, was employed for optimized biodegradation, aiming at a better understanding of how factors like pollutant initial concentration, pH, and temperature affected this process. Biodegradation reached 90 +/- 11% at 22 degrees C, pH 9.0, and 50 mg L-1. Futhermore, 8 different PAHs were biodegraded and metabolites were identified. Then, experiments with anthracene in soil ex situ were performed and bioaugmentation with Didymellaceae sp. LaBioMMi 155 presented better results than natural attenuation by the native microbiome and biostimulation by the addition of liquid nutrient medium into soil. Therefore, an expanded knowledge about PAHs biodegradation processes was achieved with emphasis to the action of Didymellaceae sp. LaBioMMi 155, which can be further employed for in situ biodegradation (after strain security test), or for enzyme identification and isolation aiming at oxygenases with optimal activity under alkaline conditions. (AU)

• FAPESP's process: 16/09690-8 - Prospecting terpenoid producers endophytic microorganisms
• Grantee: Edson Rodrigues Filho
• Support Opportunities: Regular Research Grants
• FAPESP's process: 17/19721-0 - USE OF ENVIRONMENTAL MICRORGANISMS IN THE BIODEGRADATION OF STROBILURINIC AND PYRETHROIDS PESTICIDES WIDELY EMPLOYED IN THE BRAZILIAN AGRICULTURE
• Grantee: Willian Garcia Birolli
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 19/03677-8 - Production of secondary metabolites induced by UV-A light irradiated in fungi isolated from rosemary (Rosmarinus officinalis)
• Grantee: Érika Bonatti
• Support Opportunities: Scholarships in Brazil - Scientific Initiation