| Full text | |
| Author(s): Show less - |
Scervino, Maria V. M.
;
Fortes, Marco A. S.
;
Vitzel, Kaio F.
;
de Souza, Diego R.
;
Murata, Gilson M.
;
Santana, Giovanna O.
;
da Silva, Eliane B.
;
Levada-Pires, Adriana C.
;
Kuwabara, Wilson M. T.
;
Loureiro, Tatiana C. A.
;
Curi, Rui
Total Authors: 11
|
| Document type: | Journal article |
| Source: | FEBS OPEN BIO; v. 13, n. 9, p. 14-pg., 2023-07-26. |
| Abstract | |
Autophagy plays a vital role in cell homeostasis by eliminating nonfunctional components and promoting cell survival. Here, we examined the levels of autophagy signaling proteins after 7 days of overload hypertrophy in the extensor digitorum longus (EDL) and soleus muscles of control and diabetic rats. We compared control and 3-day streptozotocin-induced diabetic rats, an experimental model for type 1 diabetes mellitus (T1DM). EDL muscles showed increased levels of basal autophagy signaling proteins. The diabetic state did not affect the extent of overload-induced hypertrophy or the levels of autophagy signaling proteins (p-ULK1, Beclin-1, Atg5, Atg12-5, Atg7, Atg3, LC3-I and II, and p62) in either muscle. The p-ULK-1, Beclin-1, and p62 protein expression levels were higher in the EDL muscle than in the soleus before the hypertrophic stimulus. On the contrary, the soleus muscle exhibited increased autophagic signaling after overload-induced hypertrophy, with increases in Beclin-1, Atg5, Atg12-5, Atg7, Atg3, and LC3-I expression in the control and diabetic groups, in addition to p-ULK-1 in the control groups. After hypertrophy, Beclin-1 and Atg5 levels increased in the EDL muscle of both groups, while p-ULK1 and LC3-I increased in the control group. In conclusion, the baseline EDL muscle exhibited higher autophagy than the soleus muscle. Although TDM1 promotes skeletal muscle mass loss and strength reduction, it did not significantly alter the extent of overload-induced hypertrophy and autophagy signaling proteins in EDL and soleus muscles, with the two groups exhibiting different patterns of autophagy activation. (AU) | |
| FAPESP's process: | 16/11661-6 - Autophagy involvement in rats skeletal muscle hypertrophy in type 1 diabetes |
| Grantee: | Maria Vitoria Martins Scervino |
| Support Opportunities: | Scholarships in Brazil - Scientific Initiation |
| FAPESP's process: | 16/15766-7 - Hypertrophy Skeletal muscle in STZ diabetic rats. |
| Grantee: | Rui Curi |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 19/19097-0 - Inflammation involvement in the hypertrophic response of the skeletal muscle in Type I Diabetic rats |
| Grantee: | Maria Vitoria Martins Scervino |
| Support Opportunities: | Scholarships in Brazil - Doctorate (Direct) |
| FAPESP's process: | 20/00707-0 - Involvement of inflammation in skeletal muscle hypertrophy and physical exercise performance |
| Grantee: | Rui Curi |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 19/02175-9 - Evaluation of synthesis and degradation of proteins in the hypertrophic response of EDL and soil muscles in rats Goto Kakizaki |
| Grantee: | Giovanna de Oliveira Santana |
| Support Opportunities: | Scholarships in Brazil - Scientific Initiation |