Glutamine supplementation accelerates functional recovery of EDL muscles after injury by modulating the expression of S100 calcium-binding proteins

Author(s)

Santos, Audrei R. ; Koike, Tatiana E. ; Santana, Alana M. ; Miranda, Natalya C. ; Dell Aquila, Rodrigo A. ; Silva, Thiago C. ; Aoki, Marcelo S. ; Miyabara, Elen H.

• Document type: Journal article
• Source: Histochemistry and Cell Biology; v. 160, n. 2, p. 12-pg., 2023-05-14.

Abstract

The aim of the current study was to investigate the effect of glutamine supplementation on the expression of HSP70 and the calcium-binding proteins from the S100 superfamily in the recovering extensor digitorum longus (EDL) muscle after injury. Two-month-old Wistar rats were subjected to cryolesion of the EDL muscle and then randomly divided into two groups (with or without glutamine supplementation). Starting immediately after the injury, the supplemented group received daily doses of glutamine (1 g/kg/day, via gavage) for 3 and 10 days orally. Then, muscles were subjected to histological, molecular, and functional analysis. Glutamine supplementation induced an increase in myofiber size of regenerating EDL muscles and prevented the decline in maximum tetanic strength of these muscles evaluated 10 days after injury. An accelerated upregulation of myogenin mRNA levels was detected in glutamine-supplemented injured muscles on day 3 post-cryolesion. The HSP70 expression increased only in the injured group supplemented with glutamine for 3 days. The increase in mRNA levels of NF-kappa B, the pro-inflammatory cytokines IL-1 beta and TNF-alpha, and the calcium-binding proteins S100A8 and S100A9 on day 3 post-cryolesion in EDL muscles was attenuated by glutamine supplementation. In contrast, the decrease in S100A1 mRNA levels in the 3-day-injured EDL muscles was minimized by glutamine supplementation. Overall, our results suggest that glutamine supplementation accelerates the recovery of myofiber size and contractile function after injury by modulating the expression of myogenin, HSP70, NF-kappa B, pro-inflammatory cytokines, and S100 calcium-binding proteins. (AU)

• FAPESP's process: 20/15351-7 - Role of beta 2 adrenoceptor in satellite cell proliferation and differentiation
• Grantee: Elen Haruka Miyabara
• Support Opportunities: Regular Research Grants
• FAPESP's process: 17/09069-4 - The influence of beta 2-adrenoceptor in satellite cell function
• Grantee: Tatiana Emy Koike
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 23/00289-2 - Expression of Set1/Trithorax-type H3K4 (histone 3 lysine 4) methyltransferases during skeletal muscle regeneration
• Grantee: Alana Moreira de Santana
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 18/24946-4 - Rehabilitation of skeletal muscle of mice with Marfan syndrome: effect of eccentric exercise
• Grantee: Elen Haruka Miyabara
• Support Opportunities: Regular Research Grants