| Full text | |
| Author(s): |
Furlan, Joao Pedro Rueda
;
Ribeiro, Teresa Gonsalves
;
Novais, Angela
;
Cabral, Hamilton
;
Peixe, Luisa
;
Stehling, Eliana Guedes
Total Authors: 6
|
| Document type: | Journal article |
| Source: | INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY; v. 73, n. 3, p. 5-pg., 2023-01-01. |
| Abstract | |
A Gram- stain-negative strain, designated BR102T, isolated from a soil sample in Brazil was characterized by a polyphasic approach. Comparative 16S rRNA gene sequences indicated that strain BR102T belonged to the genus Citrobacter. The recN- and whole-genome- based phylogeny, and multilocus sequence analysis based on concatenated partial fusA, leuS, pyrG and rpoB sequences strongly supported a clade encompassing strain BR102T and a strain from public database that was distinct from currently recognized species of the genus Citrobacter. Average nucleotide identity and digital DNA-DNA hybridization values between strain BR102T and the closest relative Citrobacter freundii ATCC 8090T were 91.8 and 48.8 %, respectively. The ability to metabolize different compounds further discriminated strain BR102T from other closely related species of the genus Citrobacter. The novel variants blaCMY-179 and qnrB97, which encoded a CMY- 2- like & beta;-lactamase and a QnrB- type protein, respec-tively, were identified in strain BR102T. BR102T was resistant to ampicillin, amoxicillin/clavulanate and cefoxitin. The DNA G+C content of strain BR102T is 51.3 mol%. Based on these results, strain BR102T represents a novel species of the genus Citrobacter, for which the name Citrobacter meridianamericanus sp. nov. is proposed. The type strain is BR102T (=MUM 22.55T=IMI 507229T). (AU) | |
| FAPESP's process: | 21/01655-7 - Resistome, plasmidome and virulome of Enterobacterales isolated from the environment carrying mcr-like genes |
| Grantee: | Eliana Guedes Stehling |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 18/01890-3 - Study of resistance, virulence and epidemiological profile of Escherichia coli isolated from environment |
| Grantee: | João Pedro Rueda Furlan |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 20/14426-3 - Cloning, expression and mapping of non-classical peptidase subsites: biotechnological application in obtaining bioactive peptides |
| Grantee: | Hamilton Cabral |
| Support Opportunities: | Regular Research Grants |