Citrobacter meridianamericanus sp. nov., isolated from a soil sample

Author(s)

Furlan, Joao Pedro Rueda ; Ribeiro, Teresa Gonsalves ; Novais, Angela ; Cabral, Hamilton ; Peixe, Luisa ; Stehling, Eliana Guedes

• Document type: Journal article
• Source: INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY; v. 73, n. 3, p. 5-pg., 2023-01-01.

Abstract

A Gram- stain-negative strain, designated BR102T, isolated from a soil sample in Brazil was characterized by a polyphasic approach. Comparative 16S rRNA gene sequences indicated that strain BR102T belonged to the genus Citrobacter. The recN- and whole-genome- based phylogeny, and multilocus sequence analysis based on concatenated partial fusA, leuS, pyrG and rpoB sequences strongly supported a clade encompassing strain BR102T and a strain from public database that was distinct from currently recognized species of the genus Citrobacter. Average nucleotide identity and digital DNA-DNA hybridization values between strain BR102T and the closest relative Citrobacter freundii ATCC 8090T were 91.8 and 48.8 %, respectively. The ability to metabolize different compounds further discriminated strain BR102T from other closely related species of the genus Citrobacter. The novel variants blaCMY-179 and qnrB97, which encoded a CMY- 2- like & beta;-lactamase and a QnrB- type protein, respec-tively, were identified in strain BR102T. BR102T was resistant to ampicillin, amoxicillin/clavulanate and cefoxitin. The DNA G+C content of strain BR102T is 51.3 mol%. Based on these results, strain BR102T represents a novel species of the genus Citrobacter, for which the name Citrobacter meridianamericanus sp. nov. is proposed. The type strain is BR102T (=MUM 22.55T=IMI 507229T). (AU)

• FAPESP's process: 21/01655-7 - Resistome, plasmidome and virulome of Enterobacterales isolated from the environment carrying mcr-like genes
• Grantee: Eliana Guedes Stehling
• Support Opportunities: Regular Research Grants
• FAPESP's process: 18/01890-3 - Study of resistance, virulence and epidemiological profile of Escherichia coli isolated from environment
• Grantee: João Pedro Rueda Furlan
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 20/14426-3 - Cloning, expression and mapping of non-classical peptidase subsites: biotechnological application in obtaining bioactive peptides
• Grantee: Hamilton Cabral
• Support Opportunities: Regular Research Grants