First genome sequence of an isolate of hibiscus chlorotic ringspot virus from the Western hemisphere

Author(s)

Ramos-Gonzalez, Pedro Luis ; da Costa-Rodrigues, Mariane ; Potsclam-Barro, Matheus ; Chabi-Jesus, Camila ; Banguela-Castillo, Alexander ; Harakava, Ricardo ; Kitajima, Elliot W. ; Freitas-Astua, Juliana

• Document type: Journal article
• Source: ROPICAL PLANT PATHOLOG; v. 45, n. 2, p. 6-pg., 2020-02-05.

Abstract

For the first time, the near-complete genome sequence of the betacarmovirus, hibiscus chlorotic ringspot virus (HCRSV) isolated from the Americas is disclosed. High throughput sequencing of the total RNA extract from a Hibiscus-rosa sinensis (L.) plant collected in Sao Paulo state, Brazil, revealed the genome sequence of HCRSV isolate SBO1, which is 3945 nucleotides long and shows 93.1% nucleotide sequence identity with HCRSV_Singapore (X86448), considered the type member of the species. These two viruses display a similar genomic organization and potentially encode seven open reading frames (ORFs). In addition, a phylogenetic analysis based on a fragment with 557 nts of p38, the coat protein gene, from a cohort of 14 samples collected in Brazil revealed a no clearly defined segregation of South American isolates from those previously detected in geographical areas outside this continent. The practical consequences of the use of p38 ORF-derived amplicons for detection and variability studies of HCRSV are concisely discussed. (AU)

• FAPESP's process: 17/50334-3 - Institutional research development plan (PDIp): modernization and adequacy of strategic multi-user units of the Instituto Biológico
• Grantee: Ana Eugênia de Carvalho Campos
• Support Opportunities: Research Grants - State Research Institutes Modernization Program
• FAPESP's process: 14/08458-9 - Plant viruses transmitted by Brevipalpus mites (Acari: Tenuipalpidae) - BTV: survey, identification, molecular characterization, phylogeny; virus/vector/host relationhip; biology, taxonomy and management of the mite
• Grantee: Elliot Watanabe Kitajima
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 17/50222-0 - Understanding the molecular biology and ecology of plant-virus-vector relationships: towards sustainable, integrated virus management strategies
• Grantee: Juliana de Freitas Astúa
• Support Opportunities: Regular Research Grants
• FAPESP's process: 16/01960-6 - Molecular characterization, phylogeny and evolución of Brevipalpus-transmitted viruses in Brazil
• Grantee: Camila Chabi de Jesus
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 19/09222-2 - Brevipalpus mite-borne virus infection in Hibiscus spp. in Brazil
• Grantee: Mariane da Costa Rodrigues
• Support Opportunities: Scholarships in Brazil - Scientific Initiation