Characterization of polymicrobial biofilms obtained from saliva or carious lesions in dentin

Author(s)

de Farias, Aline Leite ; de Carvalho, Lorena Petrucelli Freire ; Mendez, Daniela Alejandra Cusicanqui ; Cruvinel, Thiago ; Brighenti, Fernanda Lourencao

• Document type: Journal article
• Source: BIOFOULING; v. 36, n. 7, p. 11-pg., 2020-10-01.

Abstract

This study aimed to compare the formation of polymicrobial biofilms using carious dentin or saliva as inoculum for application inin vitromicrobiological studies on caries research. For biofilm growth, combined samples of infected dentin or saliva from three donors were used. The biofilms were grown on glass coverslips, under a regimen of intermittent exposure (6 h day(-1)) to 1% sucrose for 4 days. Total bacterial loads, as well as specific aciduric bacteria and mutans streptococci loads were quantified and correlated with biofilm acidogenicity and susceptibility to chlorhexidine. The data were evaluated using the Student's-t, Mann Whitney and Kruskal-Wallis tests. The two biofilms showed similar microbial loads (total bacteria, aciduric bacteria and mutans streptococci) on day 4, and high acidogenicity after 48 h and were susceptible to chlorhexidine at different time intervals. In conclusion, both dentin and saliva can be used as an inoculum inin vitrostudies of processes related to biofilm formation. (AU)

• FAPESP's process: 14/02397-8 - Interactions between cariogenic bacteria: susceptibility to chlorhexidine and Confocal Scanning Laser Microscopy using drip-flow biofilm reactor
• Grantee: Rosa Virginia Dutra de Oliveira
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• FAPESP's process: 13/12326-8 - Microbial interactions between cariogenic bacteria: evaluation of growth, metabolic activity, biochemical composition and susceptibility to chlorhexidine of biofilms formed in vitro
• Grantee: Fernanda Lourenção Brighenti
• Support Opportunities: Regular Research Grants
• FAPESP's process: 17/25904-0 - Characterization of polymicrobial biofilms from carious dentin lesions
• Grantee: Lorena Petrucelli Freire de Carvalho
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 12/17236-4 - Microbial interactions between cariogenic bacteria: evaluation of growth, metabolic activity, biochemical composition and susceptibility to chlorhexidine of biofilms formed in vitro
• Grantee: Rosa Virginia Dutra de Oliveira
• Support Opportunities: Scholarships in Brazil - Doctorate