Biotransformation of white and black mustard grains through germination and enzymatic hydrolysis revealed important metabolites for antioxidant properties and cytotoxic activity against Caco-2 cells

Author(s)

Rasera, Gabriela Boscariol ; de Vilhena Araujo, Eder ; Pereira, Alana Kelyene ; Liszbinski, Raquel Bester ; Pacheco, Guilherme ; Fill, Taicia Pacheco ; de Jesus, Marcelo Bispo ; de Castro, Ruann Janser Soares

• Document type: Journal article
• Source: Food Research International; v. 169, p. 12-pg., 2023-05-11.

Abstract

Germination and enzymatic hydrolysis are biological processes with well-recognized positive effects on phenolic composition and antioxidant potential. This study aimed to apply those processes to white (Sinapsis alba) and black (Brassica nigra) mustard grains and to analyze the influences on the total phenolic content (TPC); phenolic and peptide profile determined by ultra-performance liquid chromatography-high resolution mass spectrometry (UPLC-HRMS); antioxidant potential (DPPH, ABTS, and FRAP assays); and cytotoxicity against Caco-2, a human colorectal adenocarcinoma cell line. Enzyme combinations for hydrolysis were different for each mustard grain, but for both species, enzymatic hydrolysis and germination showed a positive effect on antioxidant properties. From UPLC-HRMS analysis and molecular network studies, 14 peptides and 17 phenolic compounds were identified as metabolites released from mustard after processes application, which were strongly correlated with increased antioxidant activity. In addition, enzymatic hydrolysis applied in germinated mustard grains for both mustards increased the cytotoxic activity against Caco-2 human colorectal adenocarcinoma cell line. (AU)

• FAPESP's process: 19/06023-9 - Investigation of molecular mechanisms induced by nanoestrutured vectors for transfection of genes in spheroid tumor cultures
• Grantee: Raquel Bester Liszbinski
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 21/12290-0 - How can germination and enzymatic hydrolysis affect the biological properties and bioaccessibility of bioactive compounds in mustard seeds?
• Grantee: Ruann Janser Soares de Castro
• Support Opportunities: Regular Research Grants