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How can germination and enzymatic hydrolysis affect the biological properties and bioaccessibility of bioactive compounds in mustard seeds?


A practice, convenient and natural diet is strongly present in the daily of people who aim a healthy lifestyle. Germination is a technique explored in many scientific researches to improve the biological, nutritional and functional characteristics of grains and seeds. The facility of execution turns this process quite convenient for people with restricted diet, that could obtain germinated products homely, as well as flours that can be used in food formulations. Thus, this project proposes the evaluation of different bioactive potentials of mustard grains disposed as: in natura, germinated and hydrolyzed by enzymes. Mustard grains, processed according to every transformation process, will be used to obtain the extracts and evaluation of their potential antioxidant, antidiabetic and antihypertensive properties. In sequence, aiming to understand the accessibility of the bioactive compounds, and consequently the form of consumption with higher benefits, in vitro simulated digestion will be performed. The final stages of the project will include the application of digested mustard extracts in intestinal cells Caco - 2 to evaluate the antioxidant activity and cytotoxicity, as well as identification and quantification of bioactive compounds (phenolics, glucosinolates and peptides) by HPLC-MS/MS and UHPLC-Q-TOF-MS. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
RASERA, GABRIELA BOSCARIOL; DE MATOS, FRANCIELLE MIRANDA; DE SOUZA SILVA, ANNA PAULA; DE ALENCAR, SEVERINO MATIAS; BRIDI, RAQUEL; COSTA DE CAMARGO, ADRIANO; SOARES DE CASTRO, RUANN JANSER. Germination and its role in phenolic compound bioaccessibility for black mustard grains: A study using INFOGEST protocol. Food Chemistry, v. 413, p. 8-pg., . (21/12290-0)
RASERA, GABRIELA BOSCARIOL; DE CAMARGO, ADRIANO COSTA; DE CASTRO, RUANN JANSER SOARES. Bioaccessibility of phenolic compounds using the standardized INFOGEST protocol: A narrative review. COMPREHENSIVE REVIEWS IN FOOD SCIENCE AND FOOD SAFETY, v. N/A, p. 27-pg., . (21/12290-0)
RASERA, GABRIELA BOSCARIOL; DE VILHENA ARAUJO, EDER; PEREIRA, ALANA KELYENE; LISZBINSKI, RAQUEL BESTER; PACHECO, GUILHERME; FILL, TAICIA PACHECO; DE JESUS, MARCELO BISPO; DE CASTRO, RUANN JANSER SOARES. Biotransformation of white and black mustard grains through germination and enzymatic hydrolysis revealed important metabolites for antioxidant properties and cytotoxic activity against Caco-2 cells. Food Research International, v. 169, p. 12-pg., . (19/06023-9, 21/12290-0)

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