Effect of flavonoid-based experimental primers on dentin microtensile bond strength and interface morphology

Author(s)

Sahadi, Beatriz Ometto ; Andre, Carolina Bosso ; Sebold, Maicon ; Giannini, Marcelo

• Document type: Journal article
• Source: INTERNATIONAL JOURNAL OF ADHESION AND ADHESIVES; v. 124, p. 9-pg., 2023-05-12.

Abstract

Objectives: This study evaluated the effect of four flavonoid-based experimental primers on the dentin bond strength (mu TBS) and the bonding interface morphology of an adhesive.Materials and methods: Four experimental primer solutions containing two different flavonoids (Kaempferol and Naringin) in two concentrations (10 mM and 20 mM) were applied for 30 s or 60 s on dentin after phosphoric acid etching, followed by an etch-and-rinse adhesive system. A Negative Control (NC) consisting of dentin not treated with experimental primer solutions and Positive Controls (PC) that used 0.2% chlorhexidine solution for 30 and 60 s were also tested. Eighty-eight teeth were selected for dentin mu TBS (n = 8). Dentin-adhesive interface morphology (n = 4) were analyzed by confocal laser scanning microscopy (CLSM). Data were analyzed by mixed three-way ANOVA (mu TBS) and two-way ANOVA (CLSM) (alpha = 0.05). Dentin mu TBS was analyzed at 24 h and one-year, while the bonding interface morphology was evaluated at 24 h.Results: Regarding mu TBS, no statistical difference was found between the experimental groups and NC at 24 h. However, most experimental primers applied for 60 s presented higher values compared to NC at one-year. Hybrid layer formation and adhesive infiltration was observed in all groups by CLSM. Conclusions: The application of flavonoid-based experimental primers for 60 s was able to produce higher mu TBS than those obtained with NC and PC after one-year of artificial aging, without interfering with interface morphology. (AU)

• FAPESP's process: 19/14973-7 - In vitro evaluation of the effect of experimental primers on dentin-adhesive bonding and dentin matrix matalloproteinase inhibition
• Grantee: Beatriz Ometto Sahadi
• Support Opportunities: Scholarships in Brazil - Master