Bovine blastocyst-like structures derived from stem cell cultures

Author(s):Show less -

Pinzon-Arteaga, Carlos A. ; Wang, Yinjuan ; Wei, Yulei ; Orsi, Ana E. Ribeiro ; Li, Leijie ; Scatolin, Giovanna ; Liu, Lizhong ; Sakurai, Masahiro ; Ye, Jianfeng ; Ming, Hao ; Yu, Leqian ; Li, Bo ; Jiang, Zongliang ; Wu, Jun

• Document type: Journal article
• Source: CELL STEM CELL; v. 30, n. 5, p. 14-pg., 2023-05-04.

Abstract

Understanding the mechanisms of blastocyst formation and implantation is critical for improving farm animal reproduction but is hampered by a limited supply of embryos. Here, we developed an efficient method to generate bovine blastocyst-like structures (termed blastoids) via assembling bovine trophoblast stem cells and expanded potential stem cells. Bovine blastoids resemble blastocysts in morphology, cell composition, single-cell transcriptomes, in vitro growth, and the ability to elicit maternal recognition of pregnancy following transfer to recipient cows. Bovine blastoids represent an accessible in vitro model for studying embryogen-esis and improving reproductive efficiency in livestock species. (AU)

• FAPESP's process: 22/02096-4 - Assessment of X chromosome inactivation status in human blastoids
• Grantee: Ana Elisa Ribeiro Orsi
• Support Opportunities: Scholarships abroad - Research Internship - Master's degree
• FAPESP's process: 20/16612-9 - Analysis of gene expression of human preimplantation embryos for the development of a strategy for maintenance of the morulas epigenetic stage
• Grantee: Ana Elisa Ribeiro Orsi
• Support Opportunities: Scholarships in Brazil - Master