Improved functional properties of the potential probiotic Lacticaseibacillus paracasei ItalPN16 growing in cheese whey

Author(s)

Pinilla, Cristian Mauricio Barreto ; Brandelli, Adriano ; Galland, Fabiana ; Spadoti, Leila Maria ; Alves, Adriana Torres Silva e

• Document type: Journal article
• Source: Letters in Applied Microbiology; v. 76, n. 7, p. 9-pg., 2023-07-03.

Abstract

The production of probiotic bacteria requires specific and expensive culture media for maintain their viability and metabolic response during gastro-intestinal transit and cell adhesion process. The aim of this study was to compare the ability of the potential probiotic Laticaseibacillus paracasei ItalPN16 to grow in plain sweet whey (SW) and acid whey (AW), evaluating changes in some probiotic properties related to the culture media. Pasteurized SW and AW were suitable media for L. paracasei growth, since counts above 9 Log CFU/ml were achieved using <50% of the total sugars in both whey samples after 48 h at 37 & DEG;C. The L. paracasei cells obtained from AW or SW cultures showed increased resistance to pH 2.5 and 3.5, higher autoaggregation, and lower cell hydrophobicity, as compared with the control of MRS. SW also improved the biofilm formation ability and cell adhesion capability to Caco-2 cells. Our results indicate that the L. paracasei adaptation to the SW conditions, inducing metabolic changes that improved its stability to acid stress, biofilm formation, autoaggregation, and cell adhesion properties, which are important functional probiotic properties. Overall, the SW could be considered as low-cost culture medium for sustainable biomass production of L. paracasei ItalPN16. (AU)

• FAPESP's process: 21/04987-0 - Biomass production of autochthonous lactic acid bacteria in culture media with different types of cheese whey and optimization of drying processes
• Grantee: Cristian Mauricio Barreto Pinilla
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 19/01896-4 - Study of the anti-inflammatory action of peptides derived from whey on glial cells
• Grantee: Fabiana Andrea Barrera Galland
• Support Opportunities: Research Grants - Young Investigators Grants