Antimicrobial Formulation of a Bacterial Nanocellulose/Propolis-Containing Photosensitizer for Biomedical Applications

Author(s)

Goncalves, Isabella Salgado ; Lima, Lais Roncalho ; Berretta, Andresa Aparecida ; Amorim, Nathaly Alcazar ; Pratavieira, Sebastiao ; Correa, Thaila Quatrini ; Resende Nogueira, Flavia Aparecida ; Barud, Hernane Silva

• Document type: Journal article
• Source: POLYMERS; v. 15, n. 4, p. 20-pg., 2023-02-01.

Abstract

With the aim of contributing to the development of more efficient materials for wound care, new topical formulations based on bacterial nanocellulose (BNC) hydrogels containing propolis were produced. Characterizations confirmed the incorporation of propolis into the BNC matrix, maintaining its structure and properties. Rheological analysis confirmed that the hydrogels showed thixotropic behavior appropriate for topical application. Chromatographic profiles showed sustained release of propolis biomarkers for at least 20 h. The formulations did not present mutagenicity. For application in photodynamic inactivation (PDI), BNC/propolis hydrogels were prepared with the photosensitizers methylene blue (MB). Spectroscopy and confocal fluorescence microscopy confirmed the interaction of MB and propolis in BNC hydrogels, as well as the formation of a new composite material. In the antibacterial assays, formulations containing MB and propolis significantly reduced Staphylococcus aureus growth. In the presence of light, BNC/MB hydrogels completely inhibited the microorganism. Therefore, the results suggest potential materials for the prevention or treatment of Staphylococcus aureus infections in wounds. (AU)

• FAPESP's process: 13/07793-6 - CEPIV - Center for Teaching, Research and Innovation in Glass
• Grantee: Edgar Dutra Zanotto
• Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC
• FAPESP's process: 17/16278-9 - Study of the genotoxicological potential and permeation assay with Caco-2 cells of metallic complexes with promising biological activities
• Grantee: Flávia Aparecida Resende Nogueira
• Support Opportunities: Regular Research Grants
• FAPESP's process: 18/25512-8 - Biocelulose-based Cell Culture Platform
• Grantee: Hernane da Silva Barud
• Support Opportunities: Regular Research Grants
• FAPESP's process: 09/54035-4 - Facility for advanced studies of biosystems and nanostructured materials
• Grantee: Igor Polikarpov
• Support Opportunities: Multi-user Equipment Program