| Full text | |
| Author(s): |
Cruz, Elisa Marozzi
;
Machado, Lucas Simoes
;
Zamproni, Laura Nicoleti
;
Bim, Larissa Valdemarin
;
Ferreira, Paula Scanavez
;
Pinto, Leonardo Alves
;
Pessan, Luiz Antonio
;
Backes, Eduardo Henrique
;
Porcionatto, Marimelia Aparecida
Total Authors: 9
|
| Document type: | Journal article |
| Source: | PHARMACEUTICS; v. 15, n. 2, p. 22-pg., 2023-02-01. |
| Abstract | |
Neuronal loss is the ultimate pathophysiologic event in central nervous system (CNS) diseases and replacing these neurons is one of the most significant challenges in regenerative medicine. Providing a suitable microenvironment for new neuron engraftment, proliferation, and synapse formation is a primary goal for 3D bioprinting. Among the various biomaterials, gelatin methacrylate (GelMA) stands out due to its Arg-Gly-Asp (RGD) domains, which assure its biocompatibility and degradation under physiological conditions. This work aimed to produce different GelMA-based bioink compositions, verify their mechanical and biological properties, and evaluate their ability to support neurogenesis. We evaluated four different GelMA-based bioink compositions; however, when it came to their biological properties, incorporating extracellular matrix components, such as Geltrex (TM), was essential to ensure human neuroprogenitor cell viability. Finally, Geltrex (TM): 8% GelMA (1:1) bioink efficiently maintained human neuroprogenitor cell stemness and supported neuronal differentiation. Interestingly, this bioink composition provides a suitable environment for murine astrocytes to de-differentiate into neural stem cells and give rise to MAP2-positive cells. (AU) | |
| FAPESP's process: | 18/12605-8 - Development of brain-on-a-chip microplataforms for in vitro modeling of the central nervous system |
| Grantee: | Marimélia Aparecida Porcionatto |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 20/12454-0 - 3D bioprinting of reactive astrocyte-derived neural stem cells |
| Grantee: | Elisa Marozzi Cruz |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 20/11374-2 - Investigation of the effects of SARS-CoV-2 infection of neurons and astrocytes on neuroinflammation using 3D bioprinted neural tissue |
| Grantee: | Eduardo Henrique Backes |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 19/27415-2 - Bioactive scaffolds with surface modification by plasma |
| Grantee: | Luiz Antonio Pessan |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 21/14327-8 - Subarachnoid haemorrhage-in-a-chip |
| Grantee: | Laura Nicoleti Zamproni |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |