Citral Modulates MMP-2 and MMP-9 Activities on Healing of Gastric Ulcers Associated with High-Fat Diet-Induced Obesity

Author(s)

Ohara, Rie ; Dario, Felipe Lima ; Emilio-Silva, Maycon Tavares ; Assuncao, Renata ; Rodrigues, Vinicius Peixoto ; Bueno, Gabriela ; Raimundo, Priscila Romano ; da Rocha, Lucia Regina Machado ; Hiruma-Lima, Clelia Akiko

• Document type: Journal article
• Source: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES; v. 24, n. 5, p. 15-pg., 2023-03-01.

Abstract

Obesity causes low-grade inflammation that results in the development of comorbidities. In people with obesity, exacerbation of gastric lesion severity and delayed healing may aggravate gastric mucosal lesions. Accordingly, we aimed to evaluate the citral effects on gastric lesion healing in eutrophic and obese animals. C57Bl/6 male mice were divided into two groups: animals fed a standard diet (SD) or high-fat diet (HFD) for 12 weeks. Gastric ulcers were induced using acetic acid (80%) in both groups. Citral (25, 100, or 300 mg/kg) was administered orally for 3 or 10 days. A vehicle-treated negative control (1% Tween 80, 10 mL/kg) and lansoprazole-treated (30 mg/kg) were also established. Lesions were macroscopically examined by quantifying regenerated tissue and ulcer areas. Matrix metalloproteinases (MMP-2 and -9) were analyzed by zymography. The ulcer base area between the two examined periods was significantly reduced in HFD 100 and 300 mg/kg citral-treated animals. In the 100 mg/kg citral-treated group, healing progression was accompanied by reduced MMP-9 activity. Accordingly, HFD could alter MMP-9 activity, delaying the initial healing phase. Although macroscopic changes were undetectable, 10-day treatment with 100 mg/kg citral exhibited improved scar tissue progression in obese animals, with reduced MMP-9 activity and modulation of MMP-2 activation. (AU)

• FAPESP's process: 18/09873-0 - Obesity induced by hypercaloric diet: evaluation of the action of Citral in metabolic inflammation, systemic and healing of gastric ulcers
• Grantee: Clélia Akiko Hiruma Lima
• Support Opportunities: Regular Research Grants
• FAPESP's process: 20/07384-2 - Can gastric healing be altered in obese animals? Evaluation of the antiulcerogenic effect of citral in mice treated with a high-fat diet
• Grantee: Felipe Lima Dario
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 20/15225-1 - Evaluation of the citral action in the intestinal permeability and metabolic endotoxemia in obese mice fed with high-fat diet
• Grantee: Maycon Tavares Emílio Silva
• Support Opportunities: Scholarships in Brazil - Doctorate